ArticleJournal of virology2009
Bluetongue virus VP6 acts early in the replication cycle and can form the basis of chimeric virus formation.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 82 citations in OpenAlex.
- Mapping of three novel linear B-cell epitopes on the VP7 protein of epizootic hemorrhagic disease virus with monoclonal antibodies.Veterinary research · 2026Article
- A Molecular and Structural Perspective on Bluetongue Virus Entry and Assembly.Pathogens (Basel, Switzerland) · 2026Review
- Article
- Rapid Quantification of Bluetongue Virus-Neutralizing Antibodies Using Bioluminescent Reporter-Expressing Viruses.Vaccines · 2025Article
- The Potential of Disabled Infectious Single Cycle (DISC) Virus Platforms for Next Generation African Swine Fever Vaccine Development.Transboundary and emerging diseases · 2025Review
- Engineering recombinant replication-competent bluetongue viruses expressing reporter genes forMicrobiology spectrum · 2024Article
- Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Reovirus RNA recombination is sequence directed and generates internally deleted defective genome segments during passage.Journal of virology · 2021Article
- Reverse Genetics Approach for Developing Rotavirus Vaccine Candidates Carrying VP4 and VP7 Genes Cloned from Clinical Isolates of Human Rotavirus.Journal of virology · 2020Article
- Reverse genetics approaches: a novel strategy for African horse sickness virus vaccine design.Current opinion in virology · 2020Review
- Differential Localization of Structural and Non-Structural Proteins during the Bluetongue Virus Replication Cycle.Viruses · 2020Article
- PCR-based reverse genetics strategy for bluetongue virus recovery.Virology journal · 2019Article
- Transcriptome analysis of responses to bluetongue virus infection in Aedes albopictus cells.BMC microbiology · 2019Article
- The Interaction of Bluetongue Virus VP6 and Genomic RNA Is Essential for Genome Packaging.Journal of virology · 2019Article
- Genome packaging in multi-segmented dsRNA viruses: distinct mechanisms with similar outcomes.Current opinion in virology · 2018Review
- A correlation between capsid protein VP2 and the plaque morphology of African horse sickness virus in cell culture.Virus genes · 2018Article
- Interaction between a Unique Minor Protein and a Major Capsid Protein of Bluetongue Virus Controls Virus Infectivity.Journal of virology · 2018Article
- Elucidating virus entry using a tetracysteine-tagged virus.Methods (San Diego, Calif.) · 2017Review
- Article
- B-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse sera.The Onderstepoort journal of veterinary research · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
A minor core protein, VP6, of bluetongue virus (BTV) possesses nucleoside triphosphatase, RNA binding, and helicase activities. Although the enzymatic functions of VP6 have been documented in vitro using purified protein, its definitive role in BTV replication remains unclear. In this study, using a recently developed T7 transcript-based reverse genetics system for BTV, we examined the importance of VP6 in virus replication. We show that VP6 is active early in replication, consistent with a role as part of the transcriptase or packaging complex, and that its action can be provided in trans by a newly developed complementary cell line. Furthermore, the genomic segment encoding VP6 was mutated to reveal the cis-acting sequences required for replication or packaging, which subsequently enabled the construction of a chimeric BTV expressing enhanced green fluorescent protein. These data confirm that one of the 10 genome segments of BTV can be replaced with a chimeric RNA containing the essential packaging and replication signals of BTV and the coding sequence of a foreign gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.