ArticleNucleic acids research2009
Separation of 1-23-kb complementary DNA strands by urea-agarose gel electrophoresis.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
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- Reverse Electrochemical Sensing of FLT3-ITD Mutations in Acute Myeloid Leukemia Using Gold Sputtered ZnO-Nanorod Configured DNA Biosensors.Biosensors · 2022Article
- Guide-free Cas9 from pathogenicScience advances · 2020Article
- Rapid in vitro production of single-stranded DNA.Nucleic acids research · 2019Article
- Near-continuously synthesized leading strands inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Endogenous single-strand DNA breaks at RNA polymerase II promoters in Saccharomyces cerevisiae.Nucleic acids research · 2018Article
- The Future of Multiplexed Eukaryotic Genome Engineering.ACS chemical biology · 2018Article
- Dual daughter strand incision is processive and increases the efficiency of DNA mismatch repair.Nucleic acids research · 2016Article
- Characterisation of the DNA sequence specificity, cellular toxicity and cross-linking properties of novel bispyridine-based dinuclear platinum complexes.BMC cancer · 2016Article
- H2AX prevents CtIP-mediated DNA end resection and aberrant repair in G1-phase lymphocytes.Nature · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Double-stranded (ds), as well as denatured, single-stranded (ss) DNA samples can be analyzed on urea-agarose gels. Here we report that after denaturation by heat in the presence of 8 M urea, the two strands of the same ds DNA fragment of approximately 1-20-kb size migrate differently in 1 M urea containing agarose gels. The two strands are readily distinguished on Southern blots by ss-specific probes. The different migration of the two strands could be attributed to their different, base composition-dependent conformation impinging on the electrophoretic mobility of the ss molecules. This phenomenon can be exploited for the efficient preparation of strand-specific probes and for the separation of the complementary DNA strands for subsequent analysis, offering a new tool for various cell biological research areas.
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