ArticleJournal of cancer research and clinical oncology2009
DNA methylation regulates constitutive expression of Stat6 regulatory genes SOCS-1 and SHP-1 in colon cancer cells.
Article in Journal of cancer research and clinical oncology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Pan-cancer analysis of the prognostic and immunological roles of SHP-1/ptpn6.Scientific reports · 2024Article
- The phosphatase Shp1 interacts with and dephosphorylates cortactin to inhibit invadopodia function.Cell communication and signaling : CCS · 2021Article
- Review
- Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells.Oncology letters · 2017Article
- SHP-1: the next checkpoint target for cancer immunotherapy?Biochemical Society transactions · 2016Review
- Effect of Regulatory Element DNA Methylation on Tissue-Type Plasminogen Activator Gene Expression.PloS one · 2016Article
- Constitutive Activation of Interleukin-13/STAT6 Contributes to Kaposi's Sarcoma-Associated Herpesvirus-Related Primary Effusion Lymphoma Cell Proliferation and Survival.Journal of virology · 2015Article
- Extensive survey of STAT6 expression in a large series of mesenchymal tumors.American journal of clinical pathology · 2015Article
- PTPN6 expression is epigenetically regulated and influences survival and response to chemotherapy in high-grade gliomas.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- The role of suppressors of cytokine signalling in human neoplasms.Molecular biology international · 2014Review
- Immune markers and differential signaling networks in ulcerative colitis and Crohn's disease.Inflammatory bowel diseases · 2012Article
- Effects of Herba epimedii and Fructus ligustri lucidi on the transcription factors in hypothalamus of aged rats.Chinese journal of integrative medicine · 2011Article
- hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas.Oncogene · 2011Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeStat6 signaling is active in cancer cells and IL-4-induced Stat6 activities or Stat6 activational phenotypes vary among cancer cells. This study aimed at investigating possible mechanism(s) involved in the formation of varying Stat6 activities/phenotypes.
methodsStat6 regulatory genes, SOCS-1 and SHP-1, were examined for mRNA expression using RT-PCR, and their promoter DNA methylation was assayed by methylation-specific PCR in Stat6-phenotyped colon cancer cell lines. DNA methylation was then verified by sequencing. RT-PCR assay and Western blotting were used to detect the expression of SOCS-1 and SHP-1 after demethylation using 5-aza-2'-deoxycytidine.
resultsCompared with Stat6(null) Caco-2 cells, Stat6(high) HT-29 cells showed decreased constitutive expression of SOCS-1 and SHP-1, which correlated with DNA hypermethylation in these genes' promoters. Interestingly, demethylation in HT-29 cells recovered the constitutive expression of SOCS-1 and SHP-1.
conclusionsThese findings suggest that DNA methylation controls the constitutive expression of negative Stat6 regulatory genes, which may affect Stat6 activities.
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