ArticleThe Journal of membrane biology2009
Interference of shot noise of open-channel current with analysis of fast gating: patchers do not (Yet) have to care.
Article in The Journal of membrane biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Binding kinetics of quaternary ammonium ions in Kcv potassium channels.Channels (Austin, Tex.) · 2024Article
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter.FEBS letters · 2017Article
- How to resolve microsecond current fluctuations in single ion channels: the power of beta distributions.Channels (Austin, Tex.) · 2015Review
- Ca2+ block and flickering both contribute to the negative slope of the IV curve in BK channels.The Journal of general physiology · 2013Article
- Modulation of enrofloxacin binding in OmpF by Mg2+ as revealed by the analysis of fast flickering single-porin current.The Journal of general physiology · 2012Article
- Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.European biophysics journal : EBJ · 2009Article
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2 authors.
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Abstract
Microsecond gating of ion channels can be evaluated by fitting beta distributions to amplitude histograms of measured time series. The shape of these histograms is determined not only by the rate constants of the gating process (in relation to the filter frequency) but also by baseline noise and shot noise, resulting from the stochastic nature of ion flow. Under normal temporal resolution, the small shot noise can be ignored. This simplification may no longer be legitimate when rate constants reach the range above 1 mus(-1). Here, the influence of shot noise is studied by means of simulated time series for several values of single-channel current of the fully open state and baseline noise. Under realistic optimal conditions (16 pA current, 1 pA noise, 50 kHz bandwidth), ignoring the shot noise leads to an underestimation of the rate constants above 1 mus(-1) by a factor of about 2.5. However, in that range, the scatter of the evaluated rate constants is at least of the same magnitude, obscuring the systematic error. The incorporation of shot noise into the analysis will become more important when amplifiers with significantly reduced noise become available.
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