ArticleInternational journal of plant genomics2009
Heterologous expression of a membrane-spanning auxin importer: implications for functional analyses of auxin transporters.
Article in International journal of plant genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 7 citations in OpenAlex.
- Structural basis of auxin binding and transport byProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Potassium Stimulation of IAA Transport Mediated by the Arabidopsis Importer AUX1 Investigated in a Heterologous Yeast System.The Journal of membrane biology · 2019Article
- What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo.International journal of molecular sciences · 2017Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Biochemical studies of plant auxin transporters in vivo are made difficult by the presence of multiple auxin transporters and auxin-interacting proteins. Furthermore, the expression level of most such transporters in plants is likely to be too low for purification and downstream functional analysis. Heterologous expression systems should address both of these issues. We have examined a number of such systems for their efficiency in expressing AUX1 from Arabidopsis thaliana. We find that a eukaryotic system based upon infection of insect cells with recombinant baculovirus provides a high level, easily scalable expression system capable of delivering a functional assay for AUX1. Furthermore, a transient transfection system in mammalian cells enables localization of AUX1 and AUX1-mediated transport of auxin to be investigated. In contrast, we were unable to utilise P. pastoris or L. lactis expression systems to reliably express AUX1.
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Registered trials
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