ArticleProceedings of the National Academy of Sciences of the United States of America2009
Protein-free small nuclear RNAs catalyze a two-step splicing reaction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 74 citations in OpenAlex.
- Intron Retention and Alzheimer's Disease (AD): A Review of Regulation Genes Implicated in AD.Genes · 2025Review
- Unleashing the potential of catalytic RNAs to combat mis-spliced transcripts.Frontiers in bioengineering and biotechnology · 2023Review
- Unique and Repeated Stwintrons (Spliceosomal Twin Introns) in the Hypoxylaceae.Journal of fungi (Basel, Switzerland) · 2022Article
- The ChloroplastCells · 2021Review
- Role of the central junction in folding topology of the protein-free human U2-U6 snRNA complex.RNA (New York, N.Y.) · 2020Article
- RNAs in the spliceosome: Insight from cryoEM structures.Wiley interdisciplinary reviews. RNA · 2019Review
- Nucleotide Modifications Decrease Innate Immune Response Induced by Synthetic Analogs of snRNAs and snoRNAs.Genes · 2018Article
- Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function.Nature chemistry · 2017Article
- Group II intron lariat: Structural insights into the spliceosome.RNA biology · 2015Review
- Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5.Biochemistry · 2014Article
- Origin of spliceosomal introns and alternative splicing.Cold Spring Harbor perspectives in biology · 2014Article
- A role for H/ACA and C/D small nucleolar RNAs in viral replication.Molecular biotechnology · 2014Article
- A day in the life of the spliceosome.Nature reviews. Molecular cell biology · 2014Review
- Post-transcriptional modifications modulate conformational dynamics in human U2-U6 snRNA complex.RNA (New York, N.Y.) · 2014Article
- Conformational heterogeneity of the protein-free human spliceosomal U2-U6 snRNA complex.RNA (New York, N.Y.) · 2013Article
- Role of small nuclear RNAs in eukaryotic gene expression.Essays in biochemistry · 2013Review
- Article
- Emerging views about the molecular structure of the spliceosomal catalytic center.RNA biology · 2012Article
- A transition-state interaction shifts nucleobase ionization toward neutrality to facilitate small ribozyme catalysis.Journal of the American Chemical Society · 2012Article
- The RNA worlds in context.Cold Spring Harbor perspectives in biology · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Pre-mRNA splicing is a crucial step in eukaryotic gene expression and is carried out by a highly complex ribonucleoprotein assembly, the spliceosome. Many fundamental aspects of spliceosomal function, including the identity of catalytic domains, remain unknown. We show that a base-paired complex of U6 and U2 small nuclear RNAs, in the absence of the approximately 200 other spliceosomal components, performs a two-step reaction with two short RNA oligonucleotides as substrates that results in the formation of a linear RNA product containing portions of both oligonucleotides. This reaction, which is chemically identical to splicing, is dependent on and occurs in proximity of sequences known to be critical for splicing in vivo. These results prove that the complex formed by U6 and U2 RNAs is a ribozyme and can potentially carry out RNA-based catalysis in the spliceosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.