ArticleProceedings of the National Academy of Sciences of the United States of America2009
DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Mechanistic understanding of UvrA damage detection and lesion hand-off to UvrB in Nucleotide Excision Repair.Nature communications · 2025Article
- A Peek Inside the Machines of Bacterial Nucleotide Excision Repair.International journal of molecular sciences · 2021Review
- Precise sequencing of single protected-DNA fragment molecules for profiling of protein distribution and assembly on DNA.Chemical science · 2021Article
- Graphene oxide enhances the specificity of the polymerase chain reaction by modifying primer-template matching.Scientific reports · 2017Article
- Conformational rearrangement of 1,2-d(GG) intrastrand cis-diammineplatinum crosslinked DNA is driven by counter-ion penetration within the minor groove of the modified site.Journal of molecular modeling · 2017Article
- ATPase activity tightly regulates RecA nucleofilaments to promote homologous recombination.Cell discovery · 2017Article
- Multiplexing Fluorescence Anisotropy Using Frequency Encoding.Analytical chemistry · 2016Article
- Characterization of a novel DNA glycosylase from S. sahachiroi involved in the reduction and repair of azinomycin B induced DNA damage.Nucleic acids research · 2016Article
- Investigation of bacterial nucleotide excision repair using single-molecule techniques.DNA repair · 2014Review
- Real-time single-molecule imaging reveals a direct interaction between UvrC and UvrB on DNA tightropes.Nucleic acids research · 2013Article
- Prokaryotic nucleotide excision repair.Cold Spring Harbor perspectives in biology · 2013Review
- Crystal structure of the UvrB dimer: insights into the nature and functioning of the UvrAB damage engagement and UvrB-DNA complexes.Nucleic acids research · 2012Article
- Dynamics of lesion processing by bacterial nucleotide excision repair proteins.Progress in molecular biology and translational science · 2012Review
- Repair of mitomycin C mono- and interstrand cross-linked DNA adducts by UvrABC: a new model.Nucleic acids research · 2010Article
- Mechanistic and biological aspects of helicase action on damaged DNA.Cell cycle (Georgetown, Tex.) · 2010Review
- Exploring damage recognition models in prokaryotic nucleotide excision repair with a benzo[a]pyrene-derived lesion in UvrB.Biochemistry · 2009Article
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
Localized DNA melting may provide a general strategy for recognition of the wide array of chemically and structurally diverse DNA lesions repaired by the nucleotide excision repair (NER) pathway. However, it is not clear what causes such DNA melting and how it is driven. Here, we show a DNA wrapping-melting model supported by results from dynamic monitoring of the key DNA-protein and protein-protein interactions involved in the early stages of the Escherichia coli NER process. Using an analytical technique involving capillary electrophoresis coupled with laser-induced fluorescence polarization, which combines a mobility shift assay with conformational analysis, we demonstrate that DNA wrapping around UvrB, mediated by UvrA, is an early event in the damage-recognition process during E. coli NER. DNA wrapping of UvrB was confirmed by Förster resonance energy transfer and fluorescence lifetime measurements. This wrapping did not occur with readily denaturable damaged DNA substrates ("bubble" DNA), suggesting that DNA wrapping of UvrB plays an important role in the induction of DNA melting around the damage site. Analysis of DNA wrapping of mutant UvrB Y96A further suggests that a cooperative interaction between DNA wrapping of UvrA(2)B and contact of the beta-hairpin of UvrB with the bulky damage moiety may be involved in the local DNA melting at the damage site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.