ArticleThe Journal of biological chemistry2009
Importin-beta is a GDP-to-GTP exchange factor of Ran: implications for the mechanism of nuclear import.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- The Sequence [RRKLPVGRS] Is a Nuclear Localization Signal for Importin 8 Binding (NLS8): A Chemical Biology and Bioinformatics Study.International journal of molecular sciences · 2025Article
- Pitstop-2 and its novel derivative RVD-127 disrupt global cell dynamics and nuclear pores integrity by direct interaction with small GTPases.Bioengineering & translational medicine · 2023Article
- Recognition motifs for importin 4 [(L)PPRS(G/P)P] and importin 5 [KP(K/Y)LV] binding, identified by bio-informatic simulation and experimentalComputational and structural biotechnology journal · 2022Article
- Bimolecular Fluorescence Complementation: Quantitative Analysis of In Cell Interaction of Nuclear Transporter Importin α with Cargo Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- DEFECTIVE EMBRYO AND MERISTEMS genes are required for cell division and gamete viability in Arabidopsis.PLoS genetics · 2021Article
- Knockdown of Ran GTPase expression inhibits the proliferation and migration of breast cancer cells.Molecular medicine reports · 2018Article
- Dual phosphorylation of Ric-8A enhances its ability to mediate G protein α subunit folding and to stimulate guanine nucleotide exchange.Science signaling · 2018Article
- Karyopherins regulate nuclear pore complex barrier and transport function.The Journal of cell biology · 2017Article
- Quantitative Proteomic Analysis of Replicative and Nonreplicative Forms Reveals Important Insights into Chromatin Biology of Trypanosoma cruzi.Molecular & cellular proteomics : MCP · 2017Article
- A pull-down procedure for the identification of unknown GEFs for small GTPases.Small GTPases · 2016Article
- Increases in Retrograde Injury Signaling Complex-Related Transcripts in Central Axons following Injury.Neural plasticity · 2016Article
- The G protein α chaperone Ric-8 as a potential therapeutic target.Molecular pharmacology · 2015Review
- Nuclear localization signals of varicella zoster virus ORF4.Virus genes · 2014Article
- Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2.Nucleic acids research · 2013Article
- Structural characterisation of the nuclear import receptor importin alpha in complex with the bipartite NLS of Prp20.PloS one · 2013Article
- Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin.The Journal of biological chemistry · 2011Article
- Regulation of nucleocytoplasmic transport in skeletal muscle.Current topics in developmental biology · 2011Review
- The nucleotide exchange factor Ric-8A is a chaperone for the conformationally dynamic nucleotide-free state of Gαi1.PloS one · 2011Article
- Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells.Chromosoma · 2010Article
- Spatial cycles in G-protein crowd control.The EMBO journal · 2010Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ran-GTP interacts strongly with importin-beta, and this interaction promotes the release of the importin-alpha-nuclear localization signal cargo from importin-beta. Ran-GDP also interacts with importin-beta, but this interaction is 4 orders of magnitude weaker than the Ran-GTP.importin-beta interaction. Here we use the yeast complement of nuclear import proteins to show that the interaction between Ran-GDP and importin-beta promotes the dissociation of GDP from Ran. The release of GDP from the Ran-GDP-importin-beta complex stabilizes the complex, which cannot be dissociated by importin-alpha. Although Ran has a higher affinity for GDP compared with GTP, Ran in complex with importin-beta has a higher affinity for GTP. This feature is responsible for the generation of Ran-GTP from Ran-GDP by importin-beta. Ran-binding protein-1 (RanBP1) activates this reaction by forming a trimeric complex with Ran-GDP and importin-beta. Importin-alpha inhibits the GDP exchange reaction by sequestering importin-beta, whereas RanBP1 restores the GDP nucleotide exchange by importin-beta by forming a tetrameric complex with importin-beta, Ran, and importin-alpha. The exchange is also inhibited by nuclear-transport factor-2 (NTF2). We suggest a mechanism for nuclear import, additional to the established RCC1 (Ran-guanine exchange factor)-dependent pathway that incorporates these results.
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