ArticleAnalytical chemistry2009
Microsphere-based rolling circle amplification microarray for the detection of DNA and proteins in a single assay.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 82 citations in OpenAlex.
- A Novel Rolling Circle Amplification-Based Detection of SARS-CoV-2 with Multi-Region Padlock Hybridization.Diagnostics (Basel, Switzerland) · 2022Article
- Ultrasensitive Detection of Attomolar Protein Concentrations by Dropcast Single Molecule Assays.Journal of the American Chemical Society · 2020Article
- Evaluation of human epidermal growth factor receptor 2 in breast cancer with a novel specific aptamer.Cellular & molecular immunology · 2017Article
- Fluorescence based fiber optic and planar waveguide biosensors. A review.Analytica chimica acta · 2016Review
- Development of a Microsphere-Based System to Facilitate Real-Time Insulin Monitoring.Journal of diabetes science and technology · 2016Article
- Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution ofTechnology · 2015Article
- Computational and experimental approaches to reveal the effects of single nucleotide polymorphisms with respect to disease diagnostics.International journal of molecular sciences · 2014Review
- Hot embossed polyethylene through-hole chips for bead-based microfluidic devices.Biosensors & bioelectronics · 2013Article
- Porous bead-based diagnostic platforms: bridging the gaps in healthcare.Sensors (Basel, Switzerland) · 2012Review
- Ultrasensitive detection of cancer biomarkers in the clinic by use of a nanostructured microfluidic array.Analytical chemistry · 2012Article
- Quantitation of femtomolar protein levels via direct readout with the electrochemical proximity assay.Journal of the American Chemical Society · 2012Article
- Particles and microfluidics merged: perspectives of highly sensitive diagnostic detection.Mikrochimica acta · 2012Article
- High-Q optical sensors for chemical and biological analysis.Analytical chemistry · 2012Review
- Diagnostic devices for isothermal nucleic acid amplification.Sensors (Basel, Switzerland) · 2012Review
- Nanostructured immunosensor for attomolar detection of cancer biomarker interleukin-8 using massively labeled superparamagnetic particles.Angewandte Chemie (International ed. in English) · 2011Article
- Ultrasensitive detection of low-abundance surface-marker protein using isothermal rolling circle amplification in a microfluidic nanoliter platform.Small (Weinheim an der Bergstrasse, Germany) · 2011Article
- Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine.Biosensors & bioelectronics · 2011Article
- Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer.The Analyst · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We describe a high-density microarray for simultaneous detection of proteins and DNA in a single test. In this system, Rolling Circle Amplification (RCA) was used as a signal amplification method for both protein and nucleic acid detection. The microsphere sensors were tested with synthetic DNA and purified recombinant protein analytes. The target DNA sequence was designed from a highly conserved gene that encodes the outer membrane protein P6 (OMP-P6) of both typeable and nontypeable strains of Haemophilus influenzae. The proinflammatory mediators IL-6 and IL-8 were selected as target proteins. Capture antibodies were first immobilized on fluorescently encoded microspheres. The microspheres were then loaded into the etched microwells of an imaging optical fiber bundle. A sandwich assay was performed for target proteins IL-6 and IL-8 using biotin-labeled secondary antibodies. Biotinylated capture DNA probes were then attached to the detection antibodies via an avidin bridge. A padlock probe, complementary to the target sequence, was subsequently hybridized to the capture probe. In the presence of the target sequence, the padlock probe was ligated, and this circular sequence was used for RCA. Following RCA, multiple fluorescently labeled signal probes were hybridized to each amplified sequence, and the microarray was imaged using an epi-fluorescence microscope. With this assay, detection limits down to 10 fM and 1 pM were achieved for proteins and target DNA, respectively. In addition to this new approach for detecting both protein and DNA in a single test using RCA, the limit of detection for IL-8 and IL-6 was improved by 3 orders of magnitude compared to similar microsphere-based assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.