ArticleTransgenic research2010
Silencing potential of viral derived RNAi constructs in Tomato leaf curl virus-AC4 gene suppression in tomato.
Article in Transgenic research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 61 citations in OpenAlex.
- Pepino Mosaic Virus in Tomato: Challenges, Control Strategies, and Future Prospects for Resistance Breeding.International journal of molecular sciences · 2025Review
- RNA Interference-Based Genetic Engineering Maize Resistant toFrontiers in plant science · 2022Article
- Tomato auxin biosynthesis/signaling is reprogrammed by the geminivirus to enhance its pathogenicity.Planta · 2020Article
- Host Plant Strategies to Combat Against Viruses Effector Proteins.Current genomics · 2020Review
- Virus and Viroid-Derived Small RNAs as Modulators of Host Gene Expression: Molecular Insights Into Pathogenesis.Frontiers in microbiology · 2020Review
- Expression of short hairpin RNA (shRNA) targeting AC2 gene of3 Biotech · 2019Article
- Review
- Systematic miRNome profiling reveals differential microRNAs in transgenic maize metabolism.Environmental sciences Europe · 2018Article
- Review
- Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review.3 Biotech · 2017Review
- Article
- Article
- Plant miRNAome and antiviral resistance: a retrospective view and prospective challenges.Virus genes · 2014Review
- Optimisation of tomato Micro-tom regeneration and selection on glufosinate/Basta and dependency of gene silencing on transgene copy number.Plant cell reports · 2013Article
- Non-coding RNAs in crop genetic modification: considerations and predictable environmental risk assessments (ERA).Molecular biotechnology · 2013Review
- Article
- Resistance to multiple viruses in transgenic tobacco expressing fused, tandem repeat, virus-derived double-stranded RNAs.Virus genes · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated viral gene suppression in an infected tomato, by transforming it with RNA inhibition (RNAi) constructs derived from same viral gene. To develop RNAi constructs, conserved sequences ranging from 21 to 200 nt of the viral target AC4 gene of various viruses causing the tomato leaf curl disease were chosen. The double-stranded (ds)RNA producing constructs carry the sense and antisense portions of these sequences and are separated by different introns behind a constitutive promoter. We compared the levels of suppression of the viral target gene by transforming four different RNAi constructs with varied arm length of dsRNA. Gene silencing levels of the viral target gene were found to be directly proportional to the arm length of the dsRNA. We observed that dsRNA derived from longer arm-length constructs generating a pool of siRNAs that were more effective in targeting gene silencing. After transformation, one of the RNAi construct having a 21 nt arm-length produced aberrant phenotypes. These phenotypic anomalies may be due to unintended ('off-target') host transcript silencing. The unintended host transcript silencing showed modest reversion in the presence of the viral target gene. The findings presented here suggest that the arm length of dsRNA capable of producing a pool of diced siRNAs is more efficient in gene silencing, the effect of off-targeting siRNA is minimized in a pool, and off-targeting silencing can be minimized in the presence of target gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.