ArticlePLoS biology2009
Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Article in PLoS biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 115 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
115 citing papers in PubMed, 1 synthesis or guideline pooled it, 190 citations in OpenAlex.
- Estimating the total number of phosphoproteins and phosphorylation sites in eukaryotic proteomes.GigaScience · 2017Pooled it
- Phosphoproteomics of WHO-Priority Fungal Pathogens: Conserved Signaling Architecture, Pathogen-Specific Outputs, and Therapeutic Vulnerabilities.Pathogens (Basel, Switzerland) · 2026Review
- Conserved Phosphorylation of the Myosin1e TH1 Domain Impacts Membrane Association and Function in Yeast and Worms.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Global comparative structural analysis of responses to protein phosphorylation.Nature communications · 2025Article
- High-throughput functional characterization of protein phosphorylation sites in yeast.Nature biotechnology · 2022Article
- Identification andmicroPublication biology · 2021Article
- Phosphoregulation of a Conserved Herpesvirus Tegument Protein by a Virally Encoded Protein Kinase in Viral Pathogenicity and Potential Linkage between Its Evolution and Viral Phylogeny.Journal of virology · 2020Article
- Article
- Review
- Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.Journal of proteomics · 2020Article
- The TOR pathway modulates cytoophidium formation inThe Journal of biological chemistry · 2019Article
- The Balancing Act of Intrinsically Disordered Proteins: Enabling Functional Diversity while Minimizing Promiscuity.Journal of molecular biology · 2019Article
- Nucleosome Remodeling by Fun30Frontiers in molecular biosciences · 2019Review
- Evaluation of functionality for serine and threonine phosphorylation with different evolutionary ages in human and mouse.BMC genomics · 2018Article
- Chemogenomic Profiling of the Fungal Pathogen Candida albicans.Antimicrobial agents and chemotherapy · 2018Article
- Mitotic-Spindle Organizing Protein MztA Mediates Septation Signaling by Suppressing the Regulatory Subunit of Protein Phosphatase 2A-ParA inFrontiers in microbiology · 2018Article
- Phosphoproteomics-Based Profiling of Kinase Activities in Cancer Cells.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Lingering single-strand breaks trigger Rad51-independent homology-directed repair of collapsed replication forks in the polynucleotide kinase/phosphatase mutant of fission yeast.PLoS genetics · 2017Article
- The Pivotal Role of Protein Phosphorylation in the Control of Yeast Central Metabolism.G3 (Bethesda, Md.) · 2017Article
- Selection maintains signaling function of a highly diverged intrinsically disordered region.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
55 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.