ArticleReproductive sciences (Thousand Oaks, Calif.)2009
A major effect of simulated microgravity on several stages of preimplantation mouse development is lethality associated with elevated phosphorylated SAPK/JNK.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- The Critical Role of YAP/BMP/ID1 Axis on Simulated Microgravity-Induced Neural Tube Defects in Human Brain Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Effects of gravity, microgravity or microgravity simulation on early mouse embryogenesis: A review of the first two space embryo studies.Mechanobiology in medicine · 2024Article
- Beyond Earth's bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space.Reproductive biology and endocrinology : RB&E · 2024Review
- Effect of microgravity on mammalian embryo development evaluated at the International Space Station.iScience · 2023Article
- Development of a new device for manipulating frozen mouse 2-cell embryos on the International Space Station.PloS one · 2022Article
- Effects of Microgravity on Early Embryonic Development and Embryonic Stem Cell Differentiation: Phenotypic Characterization and Potential Mechanisms.Frontiers in cell and developmental biology · 2021Review
- Development of mouse preimplantation embryos in space.National science review · 2020Article
- Reproductive hazards of space travel in women and men.Nature reviews. Endocrinology · 2019Review
- Phenotypic transitions enacted by simulated microgravity do not alter coherence in gene transcription profile.NPJ microgravity · 2019Article
- Effects of Gravity, Microgravity or Microgravity Simulation on Early Mammalian Development.Stem cells and development · 2018Review
- Simulated Microgravity Using a Rotary Culture System Compromises the In Vitro Development of Mouse Preantral Follicles.PloS one · 2016Article
- Toxic stress prioritizes and imbalances stem cell differentiation: implications for new biomarkers and in vitro toxicology tests.Systems biology in reproductive medicine · 2012Review
- Eomesodermin, HAND1, and CSH1 proteins are induced by cellular stress in a stress-activated protein kinase-dependent manner.Molecular reproduction and development · 2011Article
- Oxygen levels that optimize TSC culture are identified by maximizing growth rates and minimizing stress.Placenta · 2011Article
- Benzopyrene and experimental stressors cause compensatory differentiation in placental trophoblast stem cells.Systems biology in reproductive medicine · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
We tested whether microgravity affects mouse development during a period when gravity cues chick and frog embryo development. A rotating vessel developed approximately 0.1% simulated microgravity (MGS) for embryos. Microgravity simulation resulted in blocked cell accumulation in E2.5 embryos. E1.5 and E3.5 embryos showed lesser effects. For E1.5/2.5 embryos, cell accumulation block was followed by lethality at 48 hours after MGS. For E3.5 embryos, MGS blocked development without lethality but with apoptosis. E1.5-3.5 embryos from the rotational control developed lesser effects than MGS embryos. Embryonic stress-activated protein kinase (SAPK) was phosphorylated during MGS and mediated apoptosis. Increased pSAPK suggested that lethality is due to cellular stress induced by MGS, unlike the dysfunctional development after gravitational disorientation in frog and chick embryos. Thus, MGS causes lethality, a novel phenotype not often observed in microgravity or MGS. Embryonic lethality at E2.5 and apoptosis at E3.5 are associated with SAPK function, suggesting that MGS causes a general stress response that immediately affects many aspects of development. In addition, MGS and many aspects of In vitro fertilization/assisted reproductive technologies (IVF/ART) produce nonphysiological, nonevolutionary stresses that are mediated by SAPK, suggesting the primacy of this protein kinase in a wide range of mechanisms mediating negative reproductive outcomes in IVF/ART and potentially in spaceflight.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.