ArticleThe Journal of physiology2009
Calcium diffusion enhanced after cleavage of negatively charged components of brain extracellular matrix by chondroitinase ABC.
Article in The Journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 111 citations in OpenAlex.
- Neurovascular Signaling in the Perivascular Space: Biophysical Principles and Disruption in Hypertension.Hypertension (Dallas, Tex. : 1979) · 2026Review
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- Alzheimer's Tau seeds-induced pathology enhances hippocampal extracellular diffusion.Communications biology · 2025Article
- Neurons ensheathed by perineuronal nets are prone to hyperphosphorylation of tau protein in the hibernating Syrian hamster brain.Scientific reports · 2025Article
- Orchestrating the Matrix: The Role of Glial Cells and Systemic Signals in Perineuronal Net Dynamics.Neurochemical research · 2025Review
- Review
- Displacement of extracellular chloride by immobile anionic constituents of the brain's extracellular matrix.The Journal of physiology · 2025Article
- Toward Precise Modeling of Dopamine Release Kinetics: Comparison and Validation of Kinetic Models Using Voltammetry.ACS omega · 2024Article
- Astrocytes require perineuronal nets to maintain synaptic homeostasis in mice.Nature neuroscience · 2024Article
- Assessing translational applicability of perineuronal net dysfunction in Alzheimer's disease across species.Frontiers in neuroscience · 2024Article
- Astrocyte-Neuronal Communication and Its Role in Stroke.Neurochemical research · 2023Review
- Simulations of active zone structure and function at mammalian NMJs predict that loss of calcium channels alone is not sufficient to replicate LEMS effects.Journal of neurophysiology · 2023Article
- Understanding Neuropeptide Transmission in the Brain by Optical Uncaging and Release.ACS chemical neuroscience · 2023Review
- Microglia as hackers of the matrix: sculpting synapses and the extracellular space.Cellular & molecular immunology · 2021Review
- Surfactants influence polymer nanoparticle fate within the brain.Biomaterials · 2021Article
- Multiple Particle Tracking Detects Changes in Brain Extracellular Matrix and Predicts Neurodevelopmental Age.ACS nano · 2021Article
- Diurnal changes in perineuronal nets and parvalbumin neurons in the rat medial prefrontal cortex.Brain structure & function · 2021Article
- Characterizing extracellular diffusion properties using diffusion-weighted MRS of sucrose injected in mouse brain.NMR in biomedicine · 2021Article
- Modulatory Effects of Monoamines and Perineuronal Nets on Output of Cerebellar Purkinje Cells.Frontiers in neural circuits · 2021Review
- Governing Transport Principles for Nanotherapeutic Application in the Brain.Current opinion in chemical engineering · 2020Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The concentration of extracellular calcium plays a critical role in synaptic transmission and neuronal excitability as well as other physiological processes. The time course and extent of local fluctuations in the concentration of this ion largely depend on its effective diffusion coefficient (D*) and it has been speculated that fixed negative charges on chondroitin sulphate proteoglycans (CSPGs) and other components of the extracellular matrix may influence calcium diffusion because it is a divalent cation. In this study we used ion-selective microelectrodes combined with pressure ejection or iontophoresis of ions from a micropipette to quantify diffusion characteristics of neocortex and hippocampus in rat brain slices. We show that D* for calcium is less than the value predicted from the behaviour of the monovalent cation tetramethylammonium (TMA), a commonly used diffusion probe, but D* for calcium increases in both brain regions after the slices are treated with chondroitinase ABC, an enzyme that predominantly cleaves chondroitin sulphate glycans. These results suggest that CSPGs do play a role in determining the local diffusion properties of calcium in brain tissue, most likely through electrostatic interactions mediating rapid equilibrium binding. In contrast, chondroitinase ABC does not affect either the TMA diffusion or the extracellular volume fraction, indicating that the enzyme does not alter the structure of the extracellular space and that the diffusion of small monovalent cations is not affected by CSPGs in the normal brain ionic milieu. Both calcium and CSPGs are known to have many distinct roles in brain physiology, including brain repair, and our study suggests they may be functionally coupled through calcium diffusion properties.
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