ArticleJournal of the American Society for Mass Spectrometry2009
Structural analysis of sulfated glycans by sequential double-permethylation using methyl iodide and deuteromethyl iodide.
Article in Journal of the American Society for Mass Spectrometry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 64 citations in OpenAlex.
- Glycosaminoglycans as Polyelectrolytes: Charge, Interactions, and Applications.Chembiochem : a European journal of chemical biology · 2025Review
- Upregulation of Sulfated N-Glycans in Serum as Predictive Biomarkers for Early-Stage Breast Cancer.International journal of molecular sciences · 2025Article
- Minor acidic glycans: Review of focused glycomics methods.BBA advances · 2025Article
- Recent Developments and Application of Mass Spectrometry Imaging in N-Glycosylation Studies: An Overview.Mass spectrometry (Tokyo, Japan) · 2024Review
- Coupling Isotopic Shifts with Collision Cross-Section Measurements for Carbohydrate Characterization in High-Resolution Ion Mobility Separations.Analytical chemistry · 2023Article
- Enhanced Detection of ChargedAnalytical chemistry · 2023Article
- Computationally guided conversion of the specificity of E-selectin to mimic that of Siglec-8.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Sialylated and sulfated N-Glycans in MDCK and engineered MDCK cells for influenza virus studies.Scientific reports · 2022Article
- In-Depth Compositional and Structural Characterization of N-Glycans Derived from Human Urinary Exosomes.Analytical chemistry · 2019Article
- Analytical Scheme Leading to Integrated High-Sensitivity Profiling of Glycosphingolipids Together with N- and O-Glycans from One Sample.Journal of the American Society for Mass Spectrometry · 2018Article
- Towards automation of glycomic profiling of complex biological materials.Glycoconjugate journal · 2018Article
- Recent Advances in the Analysis of Complex Glycoproteins.Analytical chemistry · 2017Review
- De Novo Sequencing of Complex Mixtures of Heparan Sulfate Oligosaccharides.Analytical chemistry · 2016Article
- Comparative glycomics analysis of influenza Hemagglutinin (H5N1) produced in vaccine relevant cell platforms.Journal of proteome research · 2013Article
- High-sensitivity analytical approaches for the structural characterization of glycoproteins.Chemical reviews · 2013Review
- Priming mass spectrometry-based sulfoglycomic mapping for identification of terminal sulfated lacdiNAc glycotope.Glycoconjugate journal · 2013Article
- Analytical glycobiology at high sensitivity: current approaches and directions.Glycoconjugate journal · 2013Review
- Structural glycomic analyses at high sensitivity: a decade of progress.Annual review of analytical chemistry (Palo Alto, Calif.) · 2013Review
- Structural analysis of N- and O-glycans released from glycoproteins.Nature protocols · 2012Article
- Harnessing glycomics technologies: integrating structure with function for glycan characterization.Electrophoresis · 2012Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
MALDI mass spectrometric characterization of sulfated glycans is often challenging due to their low ionization response in the positive ion mode. Here we demonstrate a new analytical approach, allowing the measurement of sulfated glycans by substituting the sulfate group with a deuteromethyl group. Sulfated glycan samples are initially permethylated before the methanolytic cleavage of their sulfate groups. Desulfated and permethylated glycans are then subjected to another permethylation step using deuteromethyl iodide to label the hydroxyl groups resulting from methanolysis. The number of attached sulfate groups is subsequently calculated from the mass-shift resulting from the chemical cleavage of these sulfate groups. The position of the sulfate substitution is then determined by collision-induced dissociation (CID) tandem mass spectrometry of permethylated and permethylated plus deuteromethylated samples. The described approach was initially optimized and validated using linear standard glycans, while its effectiveness has also been demonstrated here for the N-glycans derived from bovine thyroid-stimulating hormone (bTSH).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.