ArticleBMC neuroscience2009
Subcellular localization of the antidepressant-sensitive norepinephrine transporter.
Article in BMC neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 44 citations in OpenAlex.
- Cell membrane cholesterol affects serotonin transporter efflux due to altered transporter oligomerization.Molecular psychiatry · 2026Article
- Release and Reuptake Sites for Norepinephrine Are Differently Distributed Across Layers of the Primary Visual Cortex in Macaque Monkeys.The Journal of comparative neurology · 2025Article
- Catecholaminergic dysfunction drives postural and locomotor deficits in a mouse model of spinal muscular atrophy.Cell reports · 2025Article
- Impairment of exocytotic transmitter release by decynium-22 through an inhibition of ion channels.Frontiers in pharmacology · 2023Article
- Norepinephrine activates βGlia · 2022Article
- A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data.Nature communications · 2022Article
- Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.Advances in experimental medicine and biology · 2021Review
- Cholesterol and the Safety Factor for Neuromuscular Transmission.International journal of molecular sciences · 2019Review
- The genetics of human personality.Genes, brain, and behavior · 2018Review
- CaJournal of chemical neuroanatomy · 2017Article
- Article
- Differential Internalization Rates and Postendocytic Sorting of the Norepinephrine and Dopamine Transporters Are Controlled by Structural Elements in the N Termini.The Journal of biological chemistry · 2016Article
- Expanding the power of recombinase-based labeling to uncover cellular diversity.Development (Cambridge, England) · 2015Article
- Sodium-dependent vitamin C transporter-2 mediates vitamin C transport at the cortical nerve terminal.Journal of neuroscience research · 2015Article
- A Role for p38 Mitogen-activated Protein Kinase-mediated Threonine 30-dependent Norepinephrine Transporter Regulation in Cocaine Sensitization and Conditioned Place Preference.The Journal of biological chemistry · 2015Article
- Pathways of polyunsaturated fatty acid utilization: implications for brain function in neuropsychiatric health and disease.Brain research · 2015Review
- Rare autism-associated variants implicate syntaxin 1 (STX1 R26Q) phosphorylation and the dopamine transporter (hDAT R51W) in dopamine neurotransmission and behaviors.EBioMedicine · 2015Article
- Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells.American journal of physiology. Cell physiology · 2014Article
- Regulated norepinephrine transporter interaction with the neurokinin-1 receptor establishes transporter subcellular localization.The Journal of biological chemistry · 2013Article
- Norepinephrine transporter variant A457P knock-in mice display key features of human postural orthostatic tachycardia syndrome.Disease models & mechanisms · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundReuptake of synaptic norepinephrine (NE) via the antidepressant-sensitive NE transporter (NET) supports efficient noradrenergic signaling and presynaptic NE homeostasis. Limited, and somewhat contradictory, information currently describes the axonal transport and localization of NET in neurons.
resultsWe elucidate NET localization in brain and superior cervical ganglion (SCG) neurons, aided by a new NET monoclonal antibody, subcellular immunoisolation techniques and quantitative immunofluorescence approaches. We present evidence that axonal NET extensively colocalizes with syntaxin 1A, and to a limited degree with SCAMP2 and synaptophysin. Intracellular NET in SCG axons and boutons also quantitatively segregates from the vesicular monoamine transporter 2 (VMAT2), findings corroborated by organelle isolation studies. At the surface of SCG boutons, NET resides in both lipid raft and non-lipid raft subdomains and colocalizes with syntaxin 1A.
conclusionOur findings support the hypothesis that SCG NET is segregated prior to transport from the cell body from proteins comprising large dense core vesicles. Once localized to presynaptic boutons, NET does not recycle via VMAT2-positive, small dense core vesicles. Finally, once NET reaches presynaptic plasma membranes, the transporter localizes to syntaxin 1A-rich plasma membrane domains, with a portion found in cholera toxin-demarcated lipid rafts. Our findings indicate that activity-dependent insertion of NET into the SCG plasma membrane derives from vesicles distinct from those that deliver NE. Moreover, NET is localized in presynaptic membranes in a manner that can take advantage of regulatory processes targeting lipid raft subdomains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.