ArticleJournal of proteome research2010
Glycoproteomic analysis of embryonic stem cells: identification of potential glycobiomarkers using lectin affinity chromatography of glycopeptides.
Article in Journal of proteome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Glycan characteristics of human heart constituent cells maintaining organ function: relatively stable glycan profiles in cellular senescence.Biogerontology · 2021Article
- Enrichment of Intact Glycopeptides Using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The quest of cell surface markers for stem cell therapy.Cellular and molecular life sciences : CMLS · 2021Review
- Global and site-specific analysis of protein glycosylation in complex biological systems with Mass Spectrometry.Mass spectrometry reviews · 2019Review
- CRISPR/Cas9 and glycomics tools forThe Journal of biological chemistry · 2019Article
- Simultaneous Quantitation of Glycoprotein Degradation and Synthesis Rates by Integrating Isotope Labeling, Chemical Enrichment, and Multiplexed Proteomics.Analytical chemistry · 2017Article
- Inhibitory activity of a Concanavalin-isolated fraction from a glucosamine-peptides reaction system against heat resistantHeliyon · 2017Article
- Glycans and glycoproteins as specific biomarkers for cancer.Analytical and bioanalytical chemistry · 2017Review
- Proteomic identification of mammalian cell surface derived glycosylphosphatidylinositol-anchored proteins through selective glycan enrichment.Proteomics · 2014Article
- Comprehensive identification of novel proteins and N-glycosylation sites in royal jelly.BMC genomics · 2014Article
- Nanotechnologies in glycoproteomics.Clinical proteomics · 2014Review
- Regulation of glycan structures in murine embryonic stem cells: combined transcript profiling of glycan-related genes and glycan structural analysis.The Journal of biological chemistry · 2012Article
- Cell surface sialylation affects binding of enterovirus 71 to rhabdomyosarcoma and neuroblastoma cells.BMC microbiology · 2012Article
- A cell surfaceome map for immunophenotyping and sorting pluripotent stem cells.Molecular & cellular proteomics : MCP · 2012Article
- Scaling down the size and increasing the throughput of glycosyltransferase assays: activity changes on stem cell differentiation.Analytical biochemistry · 2012Article
- Lectin-functionalized microchannels for characterizing pluripotent cells and early differentiation.Biomicrofluidics · 2012Article
- Phosphoproteomic analysis: an emerging role in deciphering cellular signaling in human embryonic stem cells and their differentiated derivatives.Stem cell reviews and reports · 2012Review
- Impact of stem cells in craniofacial regenerative medicine.Frontiers in physiology · 2012Article
- Pluripotent stem cell heterogeneity and the evolving role of proteomic technologies in stem cell biology.Proteomics · 2011Review
- mSEL-1L (Suppressor/enhancer Lin12-like) protein levels influence murine neural stem cell self-renewal and lineage commitment.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Numerous studies have recently focused on the identification of specific glycan biomarkers, given the important roles that protein linked glycans play, for example, during development and disease progression. The identification of protein glycobiomarkers, which are part of a very complex proteome, has involved the use of fractionation techniques such as lectin affinity chromatography. In this study, the glycoproteomic characterization of pluripotent murine embryonic stem cells (ES) and from ES cells that were differentiated into embroid bodies (EB) was performed using immobilized Concanavalin A (ConA). This procedure allowed the isolation of glycopeptides that express biantennary and hybrid N-linked structures (ConA2 fraction) as well as high mannose glycans (ConA3 fraction) that were abundant in both ES and EB stages. A total of 293 unique N-linked glycopeptide sequences (from 180 glycoproteins) were identified in the combined data sets from ES and EB cells. Of these glycopeptides, a total of 119 sequences were identified exclusively in only one of the lectin-bound fractions (24 in the ES-ConA2, 15 in the ES-ConA3, 16 in the EB-ConA2, and 64 in the EB-ConA3). Results from this study allowed the identification of individual N-glycosylation sites of proteins that express specific glycan types. The absence of some of these lectin-bound glycopeptides in a cell stage suggested that they were derived from proteins that were either expressed exclusively on a defined developmental stage or were expressed in both cell stages but carried the lectin-bound oligosaccharides in only one of them. Therefore, these lectin-bound glycopeptides can be considered as stage-specific glycobiomarkers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.