ArticleStem cells (Dayton, Ohio)2009
Nicotinamide rescues human embryonic stem cell-derived neuroectoderm from parthanatic cell death.
Article in Stem cells (Dayton, Ohio), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- ETS1 loss in mice impairs cardiac outflow tract septation via a cell migration defect autonomous to the neural crest.Human molecular genetics · 2022Article
- Epigenomic and Other Evidence for Cannabis-Induced Aging Contextualized in a Synthetic Epidemiologic Overview of Cannabinoid-Related Teratogenesis and Cannabinoid-Related Carcinogenesis.International journal of environmental research and public health · 2022Review
- Nicotinamide Promotes Formation of Retinal Organoids From Human Pluripotent Stem CellsFrontiers in cellular neuroscience · 2022Article
- Article
- Nicotinamide Inhibits Ethanol-Induced Caspase-3 and PARP-1 Over-activation and Subsequent Neurodegeneration in the Developing Mouse Cerebellum.Cerebellum (London, England) · 2018Article
- Nicotinamide alone accelerates the conversion of mouse embryonic stem cells into mature neuronal populations.PloS one · 2017Article
- Mitochondrial regulation of cell death: a phylogenetically conserved control.Microbial cell (Graz, Austria) · 2016Review
- Small-molecule-directed, efficient generation of retinal pigment epithelium from human pluripotent stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- E1B and E4 oncoproteins of adenovirus antagonize the effect of apoptosis inducing factor.Virology · 2014Article
- Retinal repair with induced pluripotent stem cells.Translational research : the journal of laboratory and clinical medicine · 2014Review
- Using human induced pluripotent stem cells to treat retinal disease.Progress in retinal and eye research · 2013Review
- SOX2-LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Neural precursor cells cultured at physiologically relevant oxygen tensions have a survival advantage following transplantation.Stem cells translational medicine · 2013Article
- Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium.Stem cells translational medicine · 2013Article
- Article
- Efficient derivation of NPCs, spinal motor neurons and midbrain dopaminergic neurons from hESCs at 3% oxygen.Nature protocols · 2011Article
- Derivation of neural precursor cells from human ES cells at 3% O(2) is efficient, enhances survival and presents no barrier to regional specification and functional differentiation.Cell death and differentiation · 2011Article
- Human ESC-derived neural crest model reveals a key role for SOX2 in sensory neurogenesis.Cell stem cell · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Abundant cell death is observed when human embryonic stem cells (hESCs) undergo neuralization, a critical first step for future cell-based therapies addressing neurodegeneration. Using hESC neuralization as an in vitro model of human development, we demonstrated that the developing neuroepithelium acquires increased susceptibility to spontaneous cell death. We found that poly(ADP-ribose) polymerase-1 (PARP1)/apoptosis-inducing factor (AIF)-mediated cell death (parthanatos) is a dominant mechanism responsible for cell loss during hESC neuralization. The demise of neural progenitor cells, at least in part, is due to decreased endogenous antioxidant defenses and enhanced reactive oxygen species leakage from mitochondria fuelled by nonphysiological culture conditions. Under such conditions, PARP1 overactivation triggered cell death through the mitochondrial-nuclear translocation of AIF. Blocking PARP1 activity with small hairpin RNA interference or nicotinamide dramatically enhanced hESC neuralization, providing optimal survival of the developing neuroepithelium. Because nicotinamide is a physiological metabolite, our results raise the possibility that neural stem/progenitor cell survival in vivo requires a metabolic niche. We argue that small natural metabolites provide a powerful physiological tool to optimize hESC differentiation compatible with the requirements of regenerative medicine.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.