ArticleStem cells (Dayton, Ohio)2009
A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells.
Article in Stem cells (Dayton, Ohio), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 72 citations in OpenAlex.
- Red blood cell differentiation using canine-induced pluripotent stem cells.Stem cells translational medicine · 2026Article
- OCT4-induced oligodendrocyte progenitor cells promote remyelination and ameliorate disease.NPJ Regenerative medicine · 2022Article
- Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin.STAR protocols · 2021Article
- OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome.Cell stem cell · 2019Article
- Pluripotent Stem Cell-Derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins.Stem cell reports · 2019Article
- Combining Induced Pluripotent Stem Cells and Genome Editing Technologies for Clinical Applications.Cell transplantation · 2018Article
- One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes.Molecular therapy. Nucleic acids · 2017Article
- Human neural progenitors derived from integration-free iPSCs for SCI therapy.Stem cell research · 2017Article
- Effects of Propofol Treatment in Neural Progenitors Derived from Human-Induced Pluripotent Stem Cells.Neural plasticity · 2017Article
- Induced Pluripotent Stem Cells Meet Genome Editing.Cell stem cell · 2016Review
- Reverse engineering human neurodegenerative disease using pluripotent stem cell technology.Brain research · 2016Review
- Human Induced Pluripotent Stem Cell NEUROG2 Dual Knockin Reporter Lines Generated by the CRISPR/Cas9 System.Stem cells and development · 2015Article
- Stem and Progenitor Cell-Derived Astroglia Therapies for Neurological Diseases.Trends in molecular medicine · 2015Review
- Advances in reprogramming-based study of neurologic disorders.Stem cells and development · 2015Review
- Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system.Journal of visualized experiments : JoVE · 2015Article
- Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus.Cell reports · 2015Article
- TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Motor neuron differentiation from pluripotent stem cells and other intermediate proliferative precursors that can be discriminated by lineage specific reporters.Stem cell reviews and reports · 2015Article
- Evaluating Electroporation and Lipofectamine Approaches for Transient and Stable Transgene Expressions in Human Fibroblasts and Embryonic Stem Cells.Cell journal · 2015Article
- Genomic editing tools to model human diseases with isogenic pluripotent stem cells.Stem cells and development · 2014Review
Corrections and comments
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Authors and funding
12 authors at 7 institutions in 1 country.
Funding
Abstract
In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. One allele of Olig2 locus was replaced by a green fluorescent protein (GFP) cassette with a targeting efficiency of 5.7%. Targeted clone R-Olig2 (like the other clones) retained pluripotency, typical hESC morphology, and a normal parental karyotype 46,XY. Most importantly, GFP expression recapitulated endogenous Olig2 expression when R-Olig2 was induced by sonic hedgehog and retinoic acid, and GFP-positive cells could be purified by fluorescence-activated cell sorting. Consistent with previous reports on rodents, early GFP-expressing cells appeared biased to a neuronal fate, whereas late GFP-expressing cells appeared biased to an oligodendrocytic fate. This was corroborated by myoblast coculture, transplantation into the rat spinal cords, and whole genome expression profiling. The present work reports an hESC reporter line generated by homologous recombination targeting a neural lineage-specific gene, which can be differentiated and sorted to obtain pure neural progenitor populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.