ArticleJournal of cellular physiology2009
Sustained epidermal growth factor receptor levels and activation by tethered ligand binding enhances osteogenic differentiation of multi-potent marrow stromal cells.
Article in Journal of cellular physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 2 citations in OpenAlex.
- Epidermal growth factor signalling pathway in endochondral ossification: an evidence-based narrative review.Annals of medicine · 2022Review
- Cellular context alters EGF-induced ERK dynamics and reveals potential crosstalk with GDF-15.Biomicrofluidics · 2022Article
- Synthetic alternatives to Matrigel.Nature reviews. Materials · 2020Article
- Article
- Biological behavior exploration of a paclitaxel-eluting poly-l-lactide-coated Mg-Zn-Y-Nd alloy intestinal stentRSC advances · 2020Article
- Biological functions of mesenchymal stem cells and clinical implications.Cellular and molecular life sciences : CMLS · 2019Review
- FOXP1 circular RNA sustains mesenchymal stem cell identity via microRNA inhibition.Nucleic acids research · 2019Article
- The extracellular domain of epithelial cell adhesion molecule (EpCAM) enhances multipotency of mesenchymal stem cells through EGFR-LIN28-LET7 signaling.The Journal of biological chemistry · 2019Article
- Epidermal growth factor enhances spinal fusion: Posterolateral lumbar fusion model on rats.Acta orthopaedica et traumatologica turcica · 2019Article
- Current approaches for modulation of the nanoscale interface in the regulation of cell behavior.Nanomedicine : nanotechnology, biology, and medicine · 2018Review
- Mesenchymal Stromal Cell-derived Extracellular Vesicles Promote Myeloid-biased Multipotent Hematopoietic Progenitor Expansion via Toll-Like Receptor Engagement.The Journal of biological chemistry · 2016Article
- Epidermal Growth Factor Tethered to β-Tricalcium Phosphate Bone Scaffolds via a High-Affinity Binding Peptide Enhances Survival of Human Mesenchymal Stem Cells/Multipotent Stromal Cells in an Immune-Competent Parafascial Implantation Assay in Mice.Stem cells translational medicine · 2016Article
- Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro.Head & face medicine · 2015Article
- Capture of endothelial cells under flow using immobilized vascular endothelial growth factor.Biomaterials · 2015Article
- Control of stem cell fate by engineering their micro and nanoenvironment.World journal of stem cells · 2015Review
- Article
- Fibronectin at select sites binds multiple growth factors and enhances their activity: expansion of the collaborative ECM-GF paradigm.The Journal of investigative dermatology · 2014Review
- Epidermal growth factor can optimize a serum-free culture system for bone marrow stem cell proliferation in a miniature pig model.In vitro cellular & developmental biology. Animal · 2013Article
- Epiregulin can promote proliferation of stem cells from the dental apical papilla via MEK/Erk and JNK signalling pathways.Cell proliferation · 2013Article
- Manipulating the lateral diffusion of surface-anchored EGF demonstrates that receptor clustering modulates phosphorylation levels.Integrative biology : quantitative biosciences from nano to macro · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Epidermal growth factor receptor (EGFR)-mediated signaling helps regulate bone development and healing through its effects on osteogenic cells. Here, we show how EGFR activity and osteogenic differentiation responses in primary human bone marrow-derived multipotent stromal cells (MSCs) are influenced by presenting covalently tethered epidermal growth factor (tEGF) on the culture substratum, a presentation mode that reduces EGFR internalization and restricts signaling to the cell surface. In both absence and presence of tEGF, MSCs increase expression levels of EGFR and its heterodimerization partner HER2 during the course of osteogenic differentiation. tEGF substrata increased levels of phosphorylated EGFR and phosphorylated extracellular regulated kinase (ERK) compared to control substrata, and these elevations were associated with a twofold enhancement of MSC alkaline phosphatase activity at day 7 and matrix mineralization at day 21. Surprisingly, addition of soluble EGF (sEGF) to cells cultured on tEGF substrata reduces osteogenic differentiation, even though EGFR signaling is more strongly activated in acute, short-term manner by sEGF treatment than by tEGF treatment. A striking concomitant result of the sEGF effects is near-complete downregulation of EGFR and HER2, demonstrating that the tEGF/EGFR interaction is dynamically reversible even though temporally sustained. Taken together, our results show that enhanced MSC osteogenic differentiation corresponds to a sustained combination of receptor expression and ligand presentation, both of which are maintained by tEGF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.