ArticleEuropean biophysics journal : EBJ2009
Analysis of protein mobilities and interactions in living cells by multifocal fluorescence fluctuation microscopy.
Article in European biophysics journal : EBJ, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Super-Resolution Microscopy of Chromatin.Genes · 2019Review
- Retrieving the intracellular topology from multi-scale protein mobility mapping in living cells.Nature communications · 2014Article
- Scanning image correlation spectroscopy.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Review
- Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Development of time-integrated multipoint moment analysis for spatially resolved fluctuation spectroscopy with high time resolution.Biophysical journal · 2011Article
- Quantitative fluorescence imaging of protein diffusion and interaction in living cells.Nature biotechnology · 2011Article
- Dissecting chromatin interactions in living cells from protein mobility maps.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates.Biophysical journal · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spatial and temporal fluctuation microscope (STFM) presented here extends the concept of a fluorescence confocal laser scanning microscope to illumination and detection along a line. The parallel multichannel acquisition of the fluorescence signal was accomplished by using a single line of an electron-multiplying charge-coupled device camera at 14 mus time resolution for detection of the fluorescence signal. The STFM system provided fast confocal imaging (30 images per second) and allowed for the spatially resolved detection of particle concentration fluctuations in fluorescence correlation spectroscopy experiments. For the application of the STFM, an approximated theoretical description of the beam geometry, the point-spread function, and the fluorescence auto- and cross-correlation functions were derived. The STFM was applied to studies of the dynamics of promyelocytic leukemia nuclear bodies, green fluorescent protein, and chromatin-remodeling complexes in living cells. The results demonstrate the unique capabilities of the STFM for characterizing the position-dependent translocations and interactions of proteins in the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.