ArticleNature structural & molecular biology2009
AID upmutants isolated using a high-throughput screen highlight the immunity/cancer balance limiting DNA deaminase activity.
Article in Nature structural & molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed.
- Human antibody heavy chain variable region exon interacts with the 3' regulatory region to form a stable somatic hypermutation center.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Somatic hypermutation patterns are shaped by both motif position and sequence grammar.The EMBO journal · 2026Article
- A default silencing mechanism restrains stress-induced genes inbioRxiv : the preprint server for biology · 2025Article
- Toward optimizing diversifying base editors for high-throughput mutational scanning studies.Nucleic acids research · 2025Article
- Transcription elongation factor ELOF1 is required for efficient somatic hypermutation and class switch recombination.Molecular cell · 2025Article
- Transcription elongation factor ELOF1 is required for efficient somatic hypermutation and class switch recombination.bioRxiv : the preprint server for biology · 2024Article
- Directed-evolution mutations enhance DNA-binding affinity and protein stability of the adenine base editor ABE8e.Cellular and molecular life sciences : CMLS · 2024Article
- Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors.Nucleic acids research · 2024Article
- Modular cytosine base editing promotes epigenomic and genomic modifications.Nucleic acids research · 2024Article
- Engineered deaminases as a key component of DNA and RNA editing tools.Molecular therapy. Nucleic acids · 2023Review
- A Rapid Antibody Enhancement Platform inACS synthetic biology · 2023Article
- The Design and Application of DNA-Editing Enzymes as Base Editors.Annual review of biochemistry · 2023Review
- AIDmut-Seq: a Three-Step Method for Detecting Protein-DNA Binding Specificity.Microbiology spectrum · 2023Article
- HMCES protects immunoglobulin genes specifically from deletions during somatic hypermutation.Genes & development · 2022Article
- Mapping single-cell-resolution cell phylogeny reveals cell population dynamics during organ development.Nature methods · 2021Article
- Controllable genome editing with split-engineered base editors.Nature chemical biology · 2021Article
- Methods and cell-based strategies to produce antibody libraries: current state.Applied microbiology and biotechnology · 2021Review
- Avidity optimization of a MAGE-A1-specific TCR with somatic hypermutation.European journal of immunology · 2021Article
- Transcription factor binding at Ig enhancers is linked to somatic hypermutation targeting.European journal of immunology · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
DNA deaminases underpin pathways in antibody diversification (AID) and anti-viral immunity (APOBEC3s). Here we show how a high-throughput bacterial papillation assay can be used to screen for AID mutants with increased catalytic activity. The upmutations focus on a small number of residues, some highlighting regions implicated in AID's substrate interaction. Many of the upmutations bring the sequence of AID closer to that of APOBEC3s. AID upmutants can yield increased antibody diversification, raising the possibility that modification of AID's specific activity might be used to regulate antibody diversification in vivo. However, upmutation of AID also led to an increased frequency of chromosomal translocations, suggesting that AID's specific activity may have been limited by the risk of genomic instability.
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Registered trials
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