ArticleVirology2009
Portal control of viral prohead expansion and DNA packaging.
Article in Virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Characterization, genomic analysis, and decontamination application of a novel Vibrio phage BUCT787 on salmon fillets.Applied microbiology and biotechnology · 2026Article
- Bacteriophage T4 genome packaging: mechanism and application.EcoSal Plus · 2025Review
- Phi29 assembly intermediates reveal how scaffold interactions with capsid protein drive capsid construction and maturation.Science advances · 2025Article
- Mechanism of Viral DNA Packaging in Phage T4 Using Single-Molecule Fluorescence Approaches.Viruses · 2024Review
- Review
- Characterization, complete genome sequencing, and CRISPR/Cas9 system-based decontamination of a novelFrontiers in microbiology · 2023Article
- Structures of a large prolate virus capsid in unexpanded and expanded states generate insights into the icosahedral virus assembly.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Tryptophan Residues Are Critical for Portal Protein Assembly and Incorporation in Bacteriophage P22.Viruses · 2022Article
- The remarkable viral portal vertex: structure and a plausible model for mechanism.Current opinion in virology · 2021Review
- Bacteriophage ICP1: A Persistent Predator ofAnnual review of virology · 2021Article
- The T4 TerL Prohead Packaging Motor Does Not Drive DNA Translocation by a Proposed Dehydration Mechanism.Viruses · 2020Article
- The large terminase DNA packaging motor grips DNA with its ATPase domain for cleavage by the flexible nuclease domain.Nucleic acids research · 2017Article
- Review
- Structure-function analysis of the DNA translocating portal of the bacteriophage T4 packaging machine.Journal of molecular biology · 2014Article
- Function and horizontal transfer of the small terminase subunit of the tailed bacteriophage Sf6 DNA packaging nanomotor.Virology · 2013Article
- Role of channel lysines and the "push through a one-way valve" mechanism of the viral DNA packaging motor.Biophysical journal · 2012Article
- Structure, assembly, and DNA packaging of the bacteriophage T4 head.Advances in virus research · 2012Review
- Three reversible and controllable discrete steps of channel gating of a viral DNA packaging motor.Biomaterials · 2011Article
- Dynamics of the T4 bacteriophage DNA packasome motor: endonuclease VII resolvase release of arrested Y-DNA substrates.The Journal of biological chemistry · 2011Article
- Bacteriophage assembly.Viruses · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Bacteriophage T4 terminase packages DNA in vitro into empty small or large proheads (esps or elps). In vivo maturation of esps yields the more stable and voluminous elps required to contain the 170 kb T4 genome. Functional proheads can be assembled containing portal-GFP fusion proteins. In the absence of terminase activity these accumulated in esps in vivo, whereas wild-type portals were found in elps. By nuclease protection assay dsDNAs of lengths 0.1, 0.2, 0.5, 5, 11, 20, 40 or 170 kb were efficiently packaged into wild-type elps in vitro, but less so into esps and gp20-GFP elps; particularly with DNAs shorter than 11 kb. However, 0.1 kb substrates were equally efficiently packaged into all types of proheads as judged by fluorescence correlation spectroscopy. These data suggest the portal controls the expansion of the major capsid protein lattice during prohead maturation, and that this expansion is necessary for DNA protection but not for packaging.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.