ArticleJournal of molecular and cellular cardiology2009
Regulation of nuclear factor of activated T cells (NFAT) in vascular endothelial cells.
Article in Journal of molecular and cellular cardiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- The machinery of healthy vasoconstriction: an overview.Pflugers Archiv : European journal of physiology · 2025Review
- Naringenin Attenuates Ox-LDL-induced Injury in HUVECs by Regulating Lipophagy Partly through the SOCE Pathway.Current pharmaceutical design · 2025Article
- Rap1A Modulates Store-Operated Calcium Entry in the Lung Endothelium: A Novel Mechanism Controlling NFAT-Mediated Vascular Inflammation and Permeability.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- CaBiomolecules · 2024Review
- Modulation of calcium signaling and metabolic pathways in endothelial cells with magnetic fields.Nanoscale advances · 2024Article
- Targeting EB3-IPAmerican journal of respiratory cell and molecular biology · 2023Article
- Role of Ion Channel Remodeling in Endothelial Dysfunction Induced by Pulmonary Arterial Hypertension.Biomolecules · 2022Review
- The role of CaScientific reports · 2020Article
- Targeting Tyrosine kinases in Renal Cell Carcinoma: "New Bullets against Old Guys".International journal of molecular sciences · 2019Review
- Maintenance of normal blood pressure is dependent on IP3R1-mediated regulation of eNOS.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Nuclear factor of activated T cells is activated in the endothelium of retinal microvessels in diabetic mice.Journal of diabetes research · 2015Article
- Article
- Genetic characterisation of Langerin gene in human immunodeficiency virus-1-infected women from Bahia, Brazil.Memorias do Instituto Oswaldo Cruz · 2014Article
- NFAT transcription factor regulation by urocortin II in cardiac myocytes and heart failure.American journal of physiology. Heart and circulatory physiology · 2014Article
- Regulation of ca(2+) signaling in pulmonary hypertension.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2013Article
- Oxytocin-stimulated NFAT transcriptional activation in human myometrial cells.Molecular endocrinology (Baltimore, Md.) · 2012Article
- A calcineurin-independent mechanism of angiogenesis inhibition by a nonimmunosuppressive cyclosporin A analog.The Journal of pharmacology and experimental therapeutics · 2011Article
- SERCA2a controls the mode of agonist-induced intracellular Ca2+ signal, transcription factor NFAT and proliferation in human vascular smooth muscle cells.Journal of molecular and cellular cardiology · 2011Article
- Downregulation of NFAT2 promotes melanogenesis in B16 melanoma cells.Anatomy & cell biology · 2010Article
- Activation of NFATc1 is directly mediated by IP3 in adult cardiac myocytes.American journal of physiology. Heart and circulatory physiology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Proteins of the NFAT family (nuclear factor of activated T cells) are Ca(2+)-sensitive transcription factors, which are involved in hypertrophic cardiovascular remodeling. Activation and nuclear translocation is mediated by dephosphorylation by the Ca(2+)-sensitive phosphatase calcineurin (CaN). We identified Ca(2+) signals that induced nuclear translocation of NFAT in cultured calf pulmonary artery endothelial (CPAE) cells using confocal fluorescence microscopy to measure simultaneously [Ca(2+)](i) and subcellular localization of NFAT-GFP (isoforms NFATc1 and NFATc3). The vasoactive agonists ATP (5 microM) or bradykinin (20 microM) in the presence of 2 mM extracellular Ca(2+) induced Ca(2+) release from the endoplasmic reticulum (ER) and activated capacitative Ca(2+) entry (CCE), which caused robust translocation of NFAT to the nucleus. This effect was sensitive to the CaN-inhibitor cyclosporin A (1 microM). Influx of extracellular Ca(2+) via CCE, but not ER Ca(2+) release was identified as the activating Ca(2+) source. NFAT was also activated by Ca(2+) influx induced by cell swelling, reverse mode Na/Ca exchange or ionomycin treatment. NFAT regulation was isoform-specific. Whereas activation of NFATc1-GFP by ATP resulted in persistent nuclear localization, NFATc3-GFP was only transiently imported into the nucleus, followed by rapid export back to the cytoplasm. Inhibition of nuclear kinases, which mediate export of NFAT via phosphorylation, or direct block of nuclear export (Leptomycin B) resulted in stable nuclear localization of NFATc3. These data demonstrate that extracellular Ca(2+) entry mediates NFAT activation. Furthermore, the regulation of nuclear localization of NFAT is isoform-specific and dependent on nuclear export processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.