ArticleCurrent biology : CB2009
Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity.
Article in Current biology : CB, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 91 citations in OpenAlex.
- An interaction between HP1 and the Chromosomal Passenger Complex Initiates Acentrosomal Spindle Assembly inbioRxiv : the preprint server for biology · 2026Article
- β-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division.Communications biology · 2026Article
- Spindle morphology changes between meiosis and mitosis driven by CK2 regulation of the Ran pathway.The Journal of cell biology · 2025Article
- Functional study of laminin subunit α5 in high-grade serous ovarian cancer.Journal of ovarian research · 2025Article
- Maternal diabetes disrupts early corticogenesis through altered mitotic gene regulation: a transcriptomic analysis.Frontiers in endocrinology · 2025Article
- Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos.Nature communications · 2024Article
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes in Xenopus laevis egg extract.Nature communications · 2023Article
- Cohesin is required for meiotic spindle assembly independent of its role in cohesion in C. elegans.PLoS genetics · 2022Article
- Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.Cell reports · 2022Article
- A celebration of the 25th anniversary of chromatin-mediated spindle assembly.Molecular biology of the cell · 2022Article
- Highway to hell-thy meiotic divisions: Chromosome passenger complex functions driven by microtubules: CPC interactions with both the chromosomes and microtubules are important for spindle assembly and function: CPC interactions with both the chromosomes and microtubules are important for spindle assembly and function.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes.The Journal of cell biology · 2021Article
- Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules.The Journal of cell biology · 2021Article
- RanGTP and importin β regulate meiosis I spindle assembly and function in mouse oocytes.The EMBO journal · 2020Article
- Direct observation of branching MT nucleation in living animal cells.The Journal of cell biology · 2019Article
- Review
- Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth.The Journal of cell biology · 2019Article
- Article
- The chromosomal basis of meiotic acentrosomal spindle assembly and function in oocytes.Chromosoma · 2017Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
During animal cell division, a gradient of GTP-bound Ran is generated around mitotic chromatin. It is generally accepted that this RanGTP gradient is essential for organizing the spindle, because it locally activates critical spindle assembly factors. Here, we show in Xenopus laevis egg extract, where the gradient is best characterized, that spindles can assemble in the absence of a RanGTP gradient. Gradient-free spindle assembly occurred around sperm nuclei but not around chromatin-coated beads and required the chromosomal passenger complex (CPC). Artificial enrichment of CPC activity within hybrid bead arrays containing both immobilized chromatin and the CPC supported local microtubule assembly even in the absence of a RanGTP gradient. We conclude that RanGTP and the CPC constitute the two major molecular signals that spatially promote microtubule polymerization around chromatin. Furthermore, we hypothesize that the two signals mainly originate from discreet physical sites on the chromosomes to localize microtubule assembly around chromatin: a RanGTP signal from any chromatin and a CPC-dependent signal predominantly generated from centromeric chromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.