ArticleJournal of controlled release : official journal of the Controlled Release Society2009
Sustained viral gene delivery through core-shell fibers.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 151 citations in OpenAlex.
- Polyurethane nanofibrous scaffolds for wound repair: from electrospinning fabrication to biological performance evaluation.BMC biotechnology · 2026Article
- Personalised Medicine and the Potential Role of Electrospinning for Targeted Immunotherapeutics in Head and Neck Cancer.Nanomaterials (Basel, Switzerland) · 2023Review
- Designing electrospun fiber platforms for efficient delivery of genetic material and genome editing tools.Advanced drug delivery reviews · 2022Review
- An investigation of alkaline phosphatase enzymatic activity after electrospinning and electrospraying.Journal of drug delivery science and technology · 2021Article
- Review
- Delivery of a mitochondria-targeted antioxidant from biocompatible, polymeric nanofibrous scaffolds.FEBS open bio · 2021Article
- Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications.Frontiers in bioengineering and biotechnology · 2020Review
- Effective Reconstruction of Functional Urethra Promoted With ICG-001 Delivery Using Core-Shell Collagen/Poly(Llactide-co-caprolactone) [P(LLA-CL)] Nanoyarn-Based Scaffold: A Study in Dog Model.Frontiers in bioengineering and biotechnology · 2020Article
- Impact of UV sterilization and short term storage on the in vitro release kinetics and bioactivity of biomolecules from electrospun scaffolds.Scientific reports · 2019Article
- Adhesive thermosensitive gels for local delivery of viral vectors.Biotechnology and bioengineering · 2019Article
- Challenges of gene delivery to the central nervous system and the growing use of biomaterial vectors.Brain research bulletin · 2019Review
- Electrospinning Nanofibers for Therapeutics Delivery.Nanomaterials (Basel, Switzerland) · 2019Review
- The Role of Electrospun Fiber Scaffolds in Stem Cell Therapy for Skin Tissue Regeneration.Med one · 2019Article
- Biomedical Applications of Electrospun Nanofibers: Drug and Nanoparticle Delivery.Pharmaceutics · 2018Review
- Poly(lactic-co-glycolic acid) devices: Production and applications for sustained protein delivery.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Programmed biomolecule delivery to enable and direct cell migration for connective tissue repair.Nature communications · 2017Article
- Biomaterial-Guided Gene Delivery for Musculoskeletal Tissue Repair.Tissue engineering. Part B, Reviews · 2017Review
- Oncolytic virus delivery: from nano-pharmacodynamics to enhanced oncolytic effect.Oncolytic virotherapy · 2017Review
- Poly(lactic acid) nanofibrous scaffolds for tissue engineering.Advanced drug delivery reviews · 2016Review
- Myocardial tissue engineering using electrospun nanofiber composites.BMB reports · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Although viral gene transfer is efficient in achieving transgene expression for tissue engineering, drawbacks of virus dissemination, toxicity and transient gene expression due to immune response have hindered its widespread application. Many tissue engineering studies thus opt to genetically engineer cells in vitro prior to their introduction in vivo. However, it would be attractive to obviate the need for in vitro manipulation by transducing the infiltrating progenitor cells in situ. This study introduces the fabrication of a virus-encapsulated electrospun fibrous scaffold to achieve sustained and localized transduction. Adenovirus encoding the gene for green fluorescent protein was efficiently encapsulated into the core of poly(epsilon-caprolactone) fibers through co-axial electrospinning and was subsequently released via a porogen-mediated process. HEK 293 cells seeded on the scaffolds expressed high level of transgene expression over a month, while cells inoculated by scaffold supernatant showed only transient expression for a week. RAW 264.7 cells cultured on the virus-encapsulated fibers produced a lower level of IL-1 beta, TNF-alpha and IFN-alpha, suggesting that the activation of macrophage cells by the viral vector was reduced when encapsulated in the core-shell PCL fibers. In demonstrating sustained and localized cell transduction, this study presents an attractive alternative mode of applying viral gene transfer for regenerative medicine.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.