ArticleBMC cell biology2009
Hydrophobic profiles of the tail anchors in SLMAP dictate subcellular targeting.
Article in BMC cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- STRIPAK, a fundamental signaling hub of eukaryotic development.Microbiology and molecular biology reviews : MMBR · 2024Review
- SLMAP3 is essential for neurulation through mechanisms involving cytoskeletal elements, ABP, and PCP.Life science alliance · 2024Article
- The Cryptococcus neoformans STRIPAK complex controls genome stability, sexual development, and virulence.PLoS pathogens · 2024Article
- Tail-anchored membrane protein SLMAP3 is essential for targeting centrosomal proteins to the nuclear envelope in skeletal myogenesis.Open biology · 2024Article
- SLMAP3 is crucial for organogenesis through mechanisms involving primary cilia formation.Open biology · 2024Article
- Deletion of Sarcolemmal Membrane-Associated Protein Isoform 3 (SLMAP3) in Cardiac Progenitors Delays Embryonic Growth of Myocardium without Affecting Hippo Pathway.International journal of molecular sciences · 2024Article
- Article
- The Green Valley ofCells · 2022Review
- STRIPAK, a Key Regulator of Fungal Development, Operates as a Multifunctional Signaling Hub.Journal of fungi (Basel, Switzerland) · 2021Article
- Association and dissociation between the mitochondrial Far complex and Atg32 regulate mitophagy.eLife · 2020Article
- psichomics: graphical application for alternative splicing quantification and analysis.Nucleic acids research · 2019Article
- Article
- Homeostatic Control of Hpo/MST Kinase Activity through Autophosphorylation-Dependent Recruitment of the STRIPAK PP2A Phosphatase Complex.Cell reports · 2017Article
- Mechanisms of Tail-Anchored Membrane Protein Targeting and Insertion.Annual review of cell and developmental biology · 2017Review
- Targeting of tail-anchored membrane proteins to subcellular organelles in Toxoplasma gondii.Traffic (Copenhagen, Denmark) · 2017Article
- STRIPAK complexes in cell signaling and cancer.Oncogene · 2016Review
- A fungal sarcolemmal membrane-associated protein (SLMAP) homolog plays a fundamental role in development and localizes to the nuclear envelope, endoplasmic reticulum, and mitochondria.Eukaryotic cell · 2015Article
- STRIPAK complexes: structure, biological function, and involvement in human diseases.The international journal of biochemistry & cell biology · 2014Review
- Tiered assembly of the yeast Far3-7-8-9-10-11 complex at the endoplasmic reticulum.The Journal of biological chemistry · 2013Article
- Contribution of topology determinants of a viral movement protein to its membrane association, intracellular traffic, and viral cell-to-cell movement.Journal of virology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTail anchored (TA) membrane proteins target subcellular structures via a C-terminal transmembrane domain and serve prominent roles in membrane fusion and vesicle transport. Sarcolemmal Membrane Associated Protein (SLMAP) possesses two alternatively spliced tail anchors (TA1 or TA2) but their specificity of subcellular targeting remains unknown.
resultsTA1 or TA2 can direct SLMAP to reticular structures including the endoplasmic reticulum (ER), whilst TA2 directs SLMAP additionally to the mitochondria. Despite the general structural similarity of SLMAP to other vesicle trafficking proteins, we found no evidence for its localization with the vesicle transport machinery or a role in vesicle transport. The predicted transmembrane region of TA2 is flanked on either side by a positively charged amino acid and is itself less hydrophobic than the transmembrane helix present in TA1. Substitution of the positively charged amino acids, in the regions flanking the transmembrane helix of TA2, with leucine did not alter its subcellular targeting. The targeting of SLMAP to the mitochondria was dependent on the hydrophobic nature of TA2 since targeting of SLMAP-TA2 was prevented by the substitution of leucine (L) for moderately hydrophobic amino acid residues within the transmembrane region. The SLMAP-TA2-4L mutant had a hydrophobic profile that was comparable to that of SLMAP-TA1 and had identical targeting properties to SLMAP-TA1.
conclusionThus the overall hydrophobicity of the two alternatively spliced TAs in SLMAP determines its subcellular targeting and TA2 predominantly directs SLMAP to the mitochondira where it may serve roles in the function of this organelle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.