ArticleJournal of cellular and molecular medicine2010
A clinically relevant model of osteoinduction: a process requiring calcium phosphate and BMP/Wnt signalling.
Article in Journal of cellular and molecular medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Who cites it
36 citing papers in PubMed, 100 citations in OpenAlex.
- Packed for Ossification: High-Density Bioprinting of hPDC Spheroids in HAMA Toward Endochondral Ossification.Advanced healthcare materials · 2026Article
- Synchronizing the Osteochondral Regeneration Process through Spatial Patterning of Stable and Hypertrophic Cartilage Organoids.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Novel GelMA/GelMA-AEMA Hydrogel Blend with Enhanced Printability as a Carrier for iPSC-Derived Chondrocytes In Vitro.Gels (Basel, Switzerland) · 2025Article
- 4D Biofabrication of Magnetically Augmented Callus Assembloid Implants Enables Rapid Endochondral Ossification via Activation of Mechanosensitive Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Callus organoids reveal distinct cartilage to bone transition mechanisms across donors and a role for biological sex.Bone research · 2025Article
- Periosteum Containing Implicit Stem Cells: A Progressive Source of Inspiration for Bone Tissue Regeneration.International journal of molecular sciences · 2024Review
- Article
- Stirred culture of cartilaginous microtissues promotes chondrogenic hypertrophy through exposure to intermittent shear stress.Bioengineering & translational medicine · 2023Article
- Differential regulation of skeletal stem/progenitor cells in distinct skeletal compartments.Frontiers in physiology · 2023Review
- Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation.International journal of molecular sciences · 2022Article
- Periosteal Skeletal Stem Cells and Their Response to Bone Injury.Frontiers in cell and developmental biology · 2022Review
- Bone Morphogenetic Proteins, Carriers, and Animal Models in the Development of Novel Bone Regenerative Therapies.Materials (Basel, Switzerland) · 2021Review
- Microcarrier Screening and Evaluation for Dynamic Expansion of Human Periosteum-Derived Progenitor Cells in a Xenogeneic Free Medium.Frontiers in bioengineering and biotechnology · 2021Article
- Single-cell characterization and metabolic profiling of in vitro cultured human skeletal progenitors with enhanced in vivo bone forming capacity.Stem cells translational medicine · 2020Article
- Developmentally Engineered Callus Organoid Bioassemblies Exhibit Predictive In Vivo Long Bone Healing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Gelatin microspheres releasing transforming growth factor drive in vitro chondrogenesis of human periosteum derived cells in micromass culture.Acta biomaterialia · 2019Article
- In Vitro Screening of Molecularly Engineered Polyethylene Glycol Hydrogels for Cartilage Tissue Engineering using Periosteum-Derived and ATDC5 Cells.International journal of molecular sciences · 2018Article
- Bone regeneration strategies: Engineered scaffolds, bioactive molecules and stem cells current stage and future perspectives.Biomaterials · 2018Review
- Animal models for bone tissue engineering and modelling disease.Disease models & mechanisms · 2018Review
- Advancing osteochondral tissue engineering: bone morphogenetic protein, transforming growth factor, and fibroblast growth factor signaling drive ordered differentiation of periosteal cells resulting in stable cartilage and bone formation in vivo.Stem cell research & therapy · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we investigated a clinically relevant model of in vivo ectopic bone formation utilizing human periosteum derived cells (HPDCs) seeded in a Collagraft carrier and explored the mechanisms by which this process is driven. Bone formation occurred after eight weeks when a minimum of one million HPDCs was loaded on Collagraft carriers and implanted subcutaneously in NMRI nu/nu mice. De novo bone matrix, mainly secreted by the HPDCs, was found juxta-proximal of the calcium phosphate (CaP) granules suggesting that CaP may have triggered the 'osteoinductive program'. Indeed, removal of the CaP granules by ethylenediaminetetraacetic acid decalcification prior to cell seeding and implantation resulted in loss of bone formation. In addition, inhibition of endogenous bone morphogenetic protein and Wnt signalling by overexpression of the secreted antagonists Noggin and Frzb, respectively, also abrogated osteoinduction. Proliferation of the engrafted HPDCs was strongly reduced in the decalcified scaffolds or when seeded with adenovirus-Noggin/Frzb transduced HPDCs indicating that cell division of the engrafted HPDCs is required for the direct bone formation cascade. These data suggest that this model of bone formation is similar to that observed during physiological intramembranous bone development and may be of importance when investigating tissue engineering strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.