ArticleMolecular microbiology2009
6S RNA binding to Esigma(70) requires a positively charged surface of sigma(70) region 4.2.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Noncanonical RNA binding of human La-related protein 6.Nucleic acids research · 2025Article
- RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria.Nucleic acids research · 2024Article
- Ms1 RNA Interacts With the RNA Polymerase Core inFrontiers in microbiology · 2022Article
- 6S RNA, a Global Regulator of Transcription.Microbiology spectrum · 2018Review
- 6S RNA Mimics B-Form DNA to Regulate Escherichia coli RNA Polymerase.Molecular cell · 2017Article
- Ribonucleoprotein particles of bacterial small non-coding RNA IsrA (IS61 or McaS) and its interaction with RNA polymerase core may link transcription to mRNA fate.Nucleic acids research · 2016Article
- Region 4 of Rhizobium etli Primary Sigma Factor (SigA) Confers Transcriptional Laxity in Escherichia coli.Frontiers in microbiology · 2016Article
- Global regulation of transcription by a small RNA: a quantitative view.Biophysical journal · 2014Article
- Identification of novel small RNAs and characterization of the 6S RNA of Coxiella burnetii.PloS one · 2014Article
- Regulation of transcription by 6S RNAs: insights from the Escherichia coli and Bacillus subtilis model systems.RNA biology · 2014Review
- Initiating nucleotide identity determines efficiency of RNA synthesis from 6S RNA templates in Bacillus subtilis but not Escherichia coli.Nucleic acids research · 2013Article
- E. coli 6S RNA release from RNA polymerase requires σ70 ejection by scrunching and is orchestrated by a conserved RNA hairpin.RNA (New York, N.Y.) · 2012Article
- Regulation of 6S RNA by pRNA synthesis is required for efficient recovery from stationary phase in E. coli and B. subtilis.Nucleic acids research · 2012Article
- Found: the elusive ANTAR transcription antiterminator.PLoS genetics · 2012Article
- Bacterial small RNA regulators: versatile roles and rapidly evolving variations.Cold Spring Harbor perspectives in biology · 2011Review
- Bacteriophage T4 MotA activator and the β-flap tip of RNA polymerase target the same set of σ70 carboxyl-terminal residues.The Journal of biological chemistry · 2011Article
- Regulation by small RNAs in bacteria: expanding frontiers.Molecular cell · 2011Review
- 6S RNA regulation of relA alters ppGpp levels in early stationary phase.Microbiology (Reading, England) · 2010Article
- Binding and release of the 6S transcriptional control RNA.RNA (New York, N.Y.) · 2010Article
- Depletion of the non-coding regulatory 6S RNA in E. coli causes a surprising reduction in the expression of the translation machinery.BMC genomics · 2010Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
6S RNA is a small, non-coding RNA that interacts with sigma(70)-RNA polymerase and downregulates transcription at many promoters during stationary phase. When bound to sigma(70)-RNA polymerase, 6S RNA is engaged in the active site of sigma(70)-RNA polymerase in a manner similar enough to promoter DNA that the RNA can serve as a template for RNA synthesis. It has been proposed that 6S RNA mimics the conformation of DNA during transcription initiation, suggesting contacts between RNA polymerase and 6S RNA or DNA may be similar. Here we demonstrate that region 4.2 of sigma(70) is critical for the interaction between 6S RNA and RNA polymerase. We define an expanded binding surface that encompasses positively charged residues throughout the recognition helix of the helix-turn-helix motif in region 4.2, in contrast to DNA binding that is largely focused on the N-terminal region of this helix. Furthermore, negatively charged residues in region 4.2 weaken binding to 6S RNA but do not similarly affect DNA binding. We propose that the binding sites for promoter DNA and 6S RNA on region 4.2 of sigma(70) are overlapping but distinct, raising interesting possibilities for how core promoter elements contribute to defining promoters that are sensitive to 6S RNA regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.