ArticleJournal of cellular and molecular medicine2009
T17b murine embryonal endothelial progenitor cells can be induced towards both proliferation and differentiation in a fibrin matrix.
Article in Journal of cellular and molecular medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Gap Junctional Interaction of Endothelial Progenitor Cells (EPC) with Endothelial Cells Induces Angiogenic Network Formation In Vitro.International journal of molecular sciences · 2025Article
- In Vivo Vascularization of Cell-Supplemented Spider Silk-Based Hydrogels in the Arteriovenous Loop Model.Biomimetics (Basel, Switzerland) · 2025Article
- Activity of the human immortalized endothelial progenitor cell line HEPC-CB.1 supporting in vitro angiogenesis.Molecular biology reports · 2020Article
- The pro-angiogenic role of hypoxia inducible factor stabilizer FG-4592 and its application in an in vivo tissue engineering chamber model.Scientific reports · 2019Article
- Improving the Angiogenic Potential of EPCs via Engineering with Synthetic Modified mRNAs.Molecular therapy. Nucleic acids · 2018Article
- VEGF promotes endothelial progenitor cell differentiation and vascular repair through connexin 43.Stem cell research & therapy · 2017Article
- Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material.BioMed research international · 2017Article
- Tissue engineering and regenerative medicine -where do we stand?Journal of cellular and molecular medicine · 2012Review
- Endothelial progenitor cells are integrated in newly formed capillaries and alter adjacent fibrovascular tissue after subcutaneous implantation in a fibrin matrix.Journal of cellular and molecular medicine · 2011Article
- The reconstruction of lung alveolus-like structure in collagen-matrigel/microcapsules scaffolds in vitro.Journal of cellular and molecular medicine · 2011Article
- Changes of anabolic processes at the cellular and molecular level in chronic wounds under topical negative pressure can be revealed by transcriptome analysis.Journal of cellular and molecular medicine · 2011Article
- Role of guanylate binding protein-1 in vascular defects associated with chronic inflammatory diseases.Journal of cellular and molecular medicine · 2011Article
- Gene expression analysis of ischaemia and reperfusion in human microsurgical free muscle tissue transfer.Journal of cellular and molecular medicine · 2011Article
- [The development of plastic surgery: retrospective view of 80 years of "Der Chirurg" (The Surgeon)].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2009Article
- Translating tissue engineering technology platforms into cancer research.Journal of cellular and molecular medicine · 2009Review
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Endothelial progenitor cells (EPC) may enhance blood vessel formation in a variety of clinical settings such as ischaemia and tumour angiogenesis as well as in tissue-engineered matrices. In the present study, we cultured a murine endothelial progenitor cell line, T17b, in vitro in cell culture as well as in an FDA-approved fibrin matrix and investigated cell proliferation, differentiation and secretion patterns of the angiogenic growth factor VEGF under hypoxia and differentiation. We show that T17b EPC remain viable for at least 8 days in the fibrin matrix where they proliferate and form clusters including lumen-like structures. Proliferation in fibrin clots overlayed with basal medium (BM) was confirmed morphologically and immunohistochemically by positive Ki67 staining, indicating mitotic activity. Significant cell proliferation and Ki-67 expression were absent when cells were incubated with dibutyryl-cAMP and retinoic acid (RA). Incubation with dibutyryl-cAMP and RA stimulated the expression of the EPC differentiation markers von Willebrand Factor (vWF) and VEGF receptor 2 (VEGFR-2), indicating successful differentiation in the fibrin clot. EPC differentiation induced by dibutyryl-cAMP and RA was confirmed in 2-D chamber slide cultures by positive vWF immunostaining, which was absent in BM controls. EPC chamber slides also displayed positive vWF staining when exposed to hypoxia under BM conditions, indicating EPC activation and differentiation could also be induced by hypoxia. Taken together, T17b EPC secrete increased levels of VEGF when submitted to either hypoxia or differentiation and can be differentiated into mature endothelial cells not only in cell and matrigel cultures but also in a fibrin matrix that is FDA approved for clinical application.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.