ArticleYeast (Chichester, England)2009
Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification.
Article in Yeast (Chichester, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 32 citations in OpenAlex.
- Insights into the translational activation mechanisms of the COX1 mRNA in yeast mitochondria.Journal of cell science · 2025Article
- Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.PLoS genetics · 2021Article
- Platelet-derived extracellular vesicles promote the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes via CXCR2 signaling.Experimental and therapeutic medicine · 2021Article
- The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3'-UTRs of yeast mitochondrial mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structure, mechanism, and regulation of mitochondrial DNA transcription initiation.The Journal of biological chemistry · 2020Review
- The DEAD-box helicase Mss116 plays distinct roles in mitochondrial ribogenesis and mRNA-specific translation.Nucleic acids research · 2017Article
- AEMBO reports · 2017Article
- A DEAD-box RNA helicase promotes thermodynamic equilibration of kinetically trapped RNA structures in vivo.RNA (New York, N.Y.) · 2016Article
- Mam33 promotes cytochrome c oxidase subunit I translation in Saccharomyces cerevisiae mitochondria.Molecular biology of the cell · 2015Article
- Mitochondrial Transcription Factor A (TFAM) Binds to RNA Containing 4-Way Junctions and Mitochondrial tRNA.PloS one · 2015Article
- The presence of an RNA:DNA hybrid that is prone to slippage promotes termination by T7 RNA polymerase.Journal of molecular biology · 2014Article
- Yeast DEAD box protein Mss116p is a transcription elongation factor that modulates the activity of mitochondrial RNA polymerase.Molecular and cellular biology · 2014Article
- The Pet309 pentatricopeptide repeat motifs mediate efficient binding to the mitochondrial COX1 transcript in yeast.RNA biology · 2014Article
- Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.Nucleic acids research · 2013Article
- Toward a molecular understanding of RNA remodeling by DEAD-box proteins.RNA biology · 2013Review
- Yeast PPR proteins, watchdogs of mitochondrial gene expression.RNA biology · 2013Article
- The mitochondrial RNA landscape of Saccharomyces cerevisiae.PloS one · 2013Article
- Mitochondrial protein synthesis, import, and assembly.Genetics · 2012Article
- High-throughput genetic identification of functionally important regions of the yeast DEAD-box protein Mss116p.Journal of molecular biology · 2011Article
- Solution structures of DEAD-box RNA chaperones reveal conformational changes and nucleic acid tethering by a basic tail.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
The abundance of mitochondrial (mt) transcripts varies under different conditions, and is thought to depend upon rates of transcription initiation, transcription termination/attenuation and RNA processing/degradation. The requirement to maintain the balance between RNA synthesis and processing may involve coordination between these processes; however, little is known about factors that regulate the activity of mtRNA polymerase (mtRNAP). Recent attempts to identify mtRNAP-protein interactions in yeast by means of a generalized tandem affinity purification (TAP) protocol were not successful, most likely because they involved a C-terminal mtRNAP-TAP fusion (which is incompatible with mtRNAP function) and because of the use of whole-cell solubilization protocols that did not preserve the integrity of mt protein complexes. Based upon the structure of T7 RNAP (to which mtRNAPs show high sequence similarity), we identified positions in yeast mtRNAP that allow insertion of a small affinity tag, confirmed the mature N-terminus, constructed a functional N-terminal TAP-mtRNAP fusion, pulled down associated proteins, and identified them by LC-MS-MS. Among the proteins found in the pull-down were a DEAD-box protein (Mss116p) and an RNA-binding protein (Pet127p). Previous genetic experiments suggested a role for these proteins in linking transcription and RNA degradation, in that a defect in the mt degradadosome could be suppressed by overexpression of either of these proteins or, independently, by mutations in either mtRNAP or its initiation factor Mtf1p. Further, we found that Mss116p inhibits transcription by mtRNAP in vitro in a steady-state reaction. Our results support the hypothesis that Mss116p and Pet127p are involved in modulation of mtRNAP activity.
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