ArticlePloS one2009
NSOM/QD-based direct visualization of CD3-induced and CD28-enhanced nanospatial coclustering of TCR and coreceptor in nanodomains in T cell activation.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- The role of peptide conformation presented by MHC in the induction of TCR triggering.Biophysical journal · 2025Article
- CD3 downregulation identifies high-avidity human CD8 T cells.Clinical and experimental immunology · 2024Article
- Factors contributing to the potency of CD8Trends in immunology · 2023Review
- Extent of MHC Clustering Regulates Selectivity and Effectiveness of T Cell Responses.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Peptide-MHC-based nanomedicines for autoimmunity function as T-cell receptor microclustering devices.Nature nanotechnology · 2017Article
- Nanoscale Relationship Between CD4 and CD25 of T Cells Visualized with NSOM/QD-Based Dual-Color Imaging System.Nanoscale research letters · 2015Article
- The coreceptor CD4 is expressed in distinct nanoclusters and does not colocalize with T-cell receptor and active protein tyrosine kinase p56lck.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Current approaches to studying membrane organization.F1000Research · 2015Review
- Core-based lipid nanoparticles as a nanoplatform for delivery of near-infrared fluorescent imaging agents.American journal of nuclear medicine and molecular imaging · 2014Article
- Microgravity inhibits resting T cell immunity in an exposure time-dependent manner.International journal of medical sciences · 2014Article
- Imaging lipid domains in cell membranes: the advent of super-resolution fluorescence microscopy.Frontiers in plant science · 2013Review
- Selective activation of antigen-experienced T cells by anti-CD3 constrained on nanoparticles.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- NSOM/QD-based visualization of GM1 serving as platforms for TCR/CD3 mediated T-cell activation.BioMed research international · 2013Article
- Quantum dots for multiplexed detection and characterisation of prostate cancer cells using a scanning near-field optical microscope.PloS one · 2012Article
- Lipid raft involvement in yeast cell growth and death.Frontiers in oncology · 2012Article
- Evidence that the density of self peptide-MHC ligands regulates T-cell receptor signaling.PloS one · 2012Article
- Nanobarcoding: detecting nanoparticles in biological samples using in situ polymerase chain reaction.International journal of nanomedicine · 2012Article
- Mechanisms controlling granule-mediated cytolytic activity of cytotoxic T lymphocytes.Immunologic research · 2011Review
- NSOM/QD-based fluorescence-topographic image fusion directly reveals nano-spatial peak-valley polarities of CD69 and CD71 activation molecules on cell-membrane fluctuations during T-cell activation.Immunology letters · 2011Article
- Membrane-bound IL-22 after de novo production in tuberculosis and anti-Mycobacterium tuberculosis effector function of IL-22+ CD4+ T cells.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
Corrections and comments
- Erratum issued
- Erratum issued
- Erratum issued
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Direct molecular imaging of nano-spatial relationship between T cell receptor (TCR)/CD3 and CD4 or CD8 co-receptor before and after activation of a primary T cell has not been reported. We have recently innovated application of near-field scanning optical microscopy (NSOM) and immune-labeling quantum dots (QD) to image Ag-specific TCR response during in vivo clonal expansion, and now up-graded the NSOM/QD-based nanotechnology through dipole-polarization and dual-color imaging. Using this imaging system scanning cell-membrane molecules at a best-optical lateral resolution, we demonstrated that CD3, CD4 or CD8 molecules were distinctly distributed as single QD-bound molecules or nano-clusters equivalent to 2-4 QD fluorescence-intensity/size on cell-membrane of un-stimulated primary T cells, and approximately 6-10% of CD3 were co-clustering with CD4 or CD8 as 70-110 nm nano-clusters without forming nano-domains. The ligation of TCR/CD3 on CD4 or CD8 T cells led to CD3 nanoscale co-clustering or interaction with CD4 or CD8 co-receptors forming 200-500 nm nano-domains or >500 nm micro-domains. Such nano-spatial co-clustering of CD3 and CD4 or CD3 and CD8 appeared to be an intrinsic event of TCR/CD3 ligation, not purely limited to MHC engagement, and be driven by Lck phosphorylation. Importantly, CD28 co-stimulation remarkably enhanced TCR/CD3 nanoscale co-clustering or interaction with CD4 co-receptor within nano- or micro-domains on the membrane. In contrast, CD28 co-stimulation did not enhance CD8 clustering or CD3-CD8 co-clustering in nano-domains although it increased molecular number and density of CD3 clustering in the enlarged nano-domains. These nanoscale findings provide new insights into TCR/CD3 interaction with CD4 or CD8 co-receptor in T-cell activation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.