ArticleNature protocols2009
Determination of viral production in aquatic sediments using the dilution-based approach.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Ski Tourism Shapes the Snow Microbiome on Ski Slopes in the Italian Central Alps.Environmental microbiology reports · 2025Article
- In situ experimental evidences for responses of abyssal benthic biota to shifts in phytodetritus compositions linked to global climate change.Global change biology · 2021Article
- New Insights Into the Virus-to-Prokaryote Ratio (VPR) in Marine Sediments.Frontiers in microbiology · 2020Article
- Viral Infections Boost Prokaryotic Biomass Production and Organic C Cycling in Hadal Trench Sediments.Frontiers in microbiology · 2019Article
- Drivers of Bacterial α- and β-Diversity Patterns and Functioning in Subsurface Hadal Sediments.Frontiers in microbiology · 2019Article
- Nontargeted virus sequence discovery pipeline and virus clustering for metagenomic data.Nature protocols · 2017Article
- Virus-mediated archaeal hecatomb in the deep seafloor.Science advances · 2016Article
- Assessing viral taxonomic composition in benthic marine ecosystems: reliability and efficiency of different bioinformatic tools for viral metagenomic analyses.Scientific reports · 2016Article
- Quantification of Viral and Prokaryotic Production Rates in Benthic Ecosystems: A Methods Comparison.Frontiers in microbiology · 2016Article
- Virus decomposition provides an important contribution to benthic deep-sea ecosystem functioning.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Counts and sequences, observations that continue to change our understanding of viruses in nature.Journal of microbiology (Seoul, Korea) · 2015Review
- Viral Impact on Prokaryotic and Microalgal Activities in the Microphytobenthic Biofilm of an Intertidal Mudflat (French Atlantic Coast).Frontiers in microbiology · 2015Article
- Impact of CO2 leakage from sub-seabed carbon dioxide capture and storage (CCS) reservoirs on benthic virus-prokaryote interactions and functions.Frontiers in microbiology · 2015Article
- Extracellular DNA can preserve the genetic signatures of present and past viral infection events in deep hypersaline anoxic basins.Proceedings. Biological sciences · 2014Article
- Viral infections stimulate the metabolism and shape prokaryotic assemblages in submarine mud volcanoes.The ISME journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses are the most abundant and dynamic biological entities in the world's ecosystems. Marine sediments, the largest biome in the world, have the potential to represent an optimal environment for viral development. To assess the viral effect on their hosts, and to understand the ecological role of the viruses in the benthic food webs and biogeochemical cycles, measurements of benthic viral production are needed. Different direct and indirect approaches have been proposed to estimate viral production in aquatic sediments, but a standardized protocol is not available yet. The method presented in this protocol relies on the short-time incubations of sediment samples with virus-free seawater, and the subsequent determination of the increase in viral abundance over time by epifluorescence microscopy. The protocol described here is highly reliable, inexpensive and easy to use. The entire procedure takes approximately 3 days to be completed, but the method allows the parallel processing of several sediment samples, which is recommended in ecological studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.