ArticleThe EMBO journal2009
Exon, intron and splice site locations in the spliceosomal B complex.
Article in The EMBO journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Discovery of a pre-mRNA structural scaffold as a contributor to the mammalian splicing code.Nucleic acids research · 2021Article
- Alternative splicing: Human disease and quantitative analysis from high-throughput sequencing.Computational and structural biotechnology journal · 2021Review
- Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome.Cold Spring Harbor perspectives in biology · 2019Review
- Translation initiation of alphavirus mRNA reveals new insights into the topology of the 48S initiation complex.Nucleic acids research · 2018Article
- Cryo-electron microscopy snapshots of the spliceosome: structural insights into a dynamic ribonucleoprotein machine.Nature structural & molecular biology · 2017Review
- Structure of a pre-catalytic spliceosome.Nature · 2017Article
- While the revolution will not be crystallized, biochemistry reigns supreme.Protein science : a publication of the Protein Society · 2017Review
- Structural and mechanistic insights into human splicing factor SF3b complex derived using an integrated approach guided by the cryo-EM density maps.RNA biology · 2016Article
- A protein map of the yeast activated spliceosome as obtained by electron microscopy.RNA (New York, N.Y.) · 2016Article
- ATPγS stalls splicing after B complex formation but prior to spliceosome activation.RNA (New York, N.Y.) · 2016Article
- A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity.Nature communications · 2016Article
- Article
- CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast.Current opinion in structural biology · 2016Review
- Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex.The EMBO journal · 2015Article
- Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5' splice site.RNA (New York, N.Y.) · 2015Article
- Mechanisms and Regulation of Alternative Pre-mRNA Splicing.Annual review of biochemistry · 2015Review
- The spliceosome: disorder and dynamics defined.Current opinion in structural biology · 2014Review
- Structural analyses of the pre-mRNA splicing machinery.Protein science : a publication of the Protein Society · 2013Review
- Rearrangements within human spliceosomes captured after exon ligation.RNA (New York, N.Y.) · 2013Article
- Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex.RNA (New York, N.Y.) · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, electron microscopy (EM) has allowed the generation of three-dimensional structure maps of several spliceosomal complexes. However, owing to their limited resolution, little is known at present about the location of the pre-mRNA, the spliceosomal small nuclear ribonucleoprotein or the spliceosome's active site within these structures. In this work, we used EM to localise the intron and the 5' and 3' exons of a model pre-mRNA, as well as the U2-associated protein SF3b155, in pre-catalytic spliceosomes (i.e. B complexes) by labelling them with an antibody that bears colloidal gold. Our data reveal that the intron and both exons, together with SF3b155, are located in specific regions of the head domain of the B complex. These results represent an important first step towards identifying functional sites in the spliceosome. The gold-labelling method adopted here can be applied to other spliceosomal complexes and may thus contribute significantly to our overall understanding of the pre-mRNA splicing process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.