ArticleMolecular biology of the cell2009
The role of the exocyst in matrix metalloproteinase secretion and actin dynamics during tumor cell invadopodia formation.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 94 citations in OpenAlex.
- METTL3 regulates exocytosis independently of mScience advances · 2026Article
- EXOC4 Promotes Diffuse-Type Gastric Cancer Metastasis via Activating FAK Signal.Molecular cancer research : MCR · 2022Article
- Review
- TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia.Communications biology · 2021Article
- Article
- Loss of the exocyst complex component EXOC3 promotes hemostasis and accelerates arterial thrombosis.Blood advances · 2021Article
- GIV/Girdin and Exo70 Collaboratively Regulate the Mammalian Polarized Exocytic Machinery.iScience · 2020Article
- ULK1 phosphorylates Exo70 to suppress breast cancer metastasis.Nature communications · 2020Article
- A family affair: A Ral-exocyst-centered network links Ras, Rac, Rho signaling to control cell migration.Small GTPases · 2019Review
- The role of Exo70 in exocytosis and beyond.Small GTPases · 2019Review
- The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.Molecular neurobiology · 2019Article
- The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.The Journal of cell biology · 2019Article
- Review
- Identification and Characterization of theInternational journal of molecular sciences · 2018Article
- Regulation of Cell Polarity by Exocyst-Mediated Trafficking.Cold Spring Harbor perspectives in biology · 2018Review
- A Salutary Role of Reactive Oxygen Species in Intercellular Tunnel-Mediated Communication.Frontiers in cell and developmental biology · 2018Review
- Invadosomes are coming: new insights into function and disease relevance.The FEBS journal · 2018Review
- Polarized Exocytosis.Cold Spring Harbor perspectives in biology · 2017Review
- Exo70 is an independent prognostic factor in colon cancer.Scientific reports · 2017Article
- Linking cortical microtubule attachment and exocytosis.F1000Research · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Invadopodia are actin-rich membrane protrusions formed by tumor cells that degrade the extracellular matrix for invasion. Invadopodia formation involves membrane protrusions driven by Arp2/3-mediated actin polymerization and secretion of matrix metalloproteinases (MMPs) at the focal degrading sites. The exocyst mediates the tethering of post-Golgi secretory vesicles at the plasma membrane for exocytosis and has recently been implicated in regulating actin dynamics during cell migration. Here, we report that the exocyst plays a pivotal role in invadopodial activity. With RNAi knockdown of the exocyst component Exo70 or Sec8, MDA-MB-231 cells expressing constitutively active c-Src failed to form invadopodia. On the other hand, overexpression of Exo70 promoted invadopodia formation. Disrupting the exocyst function by siEXO70 or siSEC8 treatment or by expression of a dominant negative fragment of Exo70 inhibited the secretion of MMPs. We have also found that the exocyst interacts with the Arp2/3 complex in cells with high invasion potential; blocking the exocyst-Arp2/3 interaction inhibited Arp2/3-mediated actin polymerization and invadopodia formation. Together, our results suggest that the exocyst plays important roles in cell invasion by mediating the secretion of MMPs at focal degrading sites and regulating Arp2/3-mediated actin dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.