ArticleThe Journal of biological chemistry2009
Torpedo nuclease Rat1 is insufficient to terminate RNA polymerase II in vitro.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- A spontaneous termination mechanism of RNA polymerase V shapes the DNA methylation landscape in plants.The EMBO journal · 2026Article
- Article
- DNA-directed termination of RNA polymerase II transcription.Molecular cell · 2023Article
- Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF.Nature communications · 2022Article
- FttA is a CPSF73 homologue that terminates transcription in Archaea.Nature microbiology · 2020Article
- The torpedo effect in Bacillus subtilis: RNase J1 resolves stalled transcription complexes.The EMBO journal · 2020Article
- Writing a wrong: Coupled RNA polymerase II transcription and RNA quality control.Wiley interdisciplinary reviews. RNA · 2019Review
- CDK regulation of transcription by RNAP II: Not over 'til it's over?Transcription · 2017Article
- Transcription Dynamics in Living Cells.Annual review of biophysics · 2016Review
- Transcriptional termination in mammals: Stopping the RNA polymerase II juggernaut.Science (New York, N.Y.) · 2016Review
- Structures of E. coli σS-transcription initiation complexes provide new insights into polymerase mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Recent advances in understanding transcription termination by RNA polymerase II.F1000Research · 2016Review
- P-TEFb regulation of transcription termination factor Xrn2 revealed by a chemical genetic screen for Cdk9 substrates.Genes & development · 2016Article
- Unraveling the mechanistic features of RNA polymerase II termination by the 5'-3' exoribonuclease Rat1.Nucleic acids research · 2015Article
- Transcription termination and the control of the transcriptome: why, where and how to stop.Nature reviews. Molecular cell biology · 2015Review
- Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle.Nucleic acids research · 2015Article
- Fail-safe transcription termination: Because one is never enough.RNA biology · 2015Review
- The RNA exosome promotes transcription termination of backtracked RNA polymerase II.Nature structural & molecular biology · 2014Article
- Dismantling promoter-driven RNA polymerase II transcription complexes in vitro by the termination factor Rat1.The Journal of biological chemistry · 2013Article
- RNA polymerase II mutations conferring defects in poly(A) site cleavage and termination in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Termination of RNA polymerase (pol) II transcription in vivo requires the 5'-RNA exonuclease Rat1. It was proposed that Rat1 degrades RNA from the 5'-end that is created by transcript cleavage, catches up with elongating pol II, and acts like a Torpedo that removes pol II from DNA. Here we test the Torpedo model in an in vitro system based on bead-coupled pol II elongation complexes (ECs). Recombinant Rat1 complexes with Rai1, and with Rai1 and Rtt103, degrade RNA extending from the EC until they reach the polymerase surface but fail to terminate pol II. Instead, the EC retains an approximately 18-nucleotide RNA that remains with its 3'-end at the active site and can be elongated. Thus, pol II termination apparently requires a factor or several factors in addition to Rat1, Rai1, and Rtt103, post-translational modifications of these factors, or unusual reaction conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.