ArticleBMC cell biology2009
Characterization of sequences in human TWIST required for nuclear localization.
Article in BMC cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Mechanical force application to the nucleus regulates nucleocytoplasmic transport.Nature cell biology · 2022Article
- Epithelial to Mesenchymal Transition: A Challenging Playground for Translational Research. Current Models and Focus onInternational journal of molecular sciences · 2021Review
- Article
- TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation.eLife · 2021Article
- Targeted cell imaging properties of a deep red luminescent iridium(iii) complex conjugated with a c-Myc signal peptide.Chemical science · 2020Article
- Emerging role of Twist1 in fibrotic diseases.Journal of cellular and molecular medicine · 2018Review
- N-terminal truncations of human bHLH transcription factor Twist1 leads to the formation of aggresomes.Molecular and cellular biochemistry · 2018Article
- Novel Molecular Markers for Breast Cancer.Biomarkers in cancer · 2016Review
- Article
- PRMT1 Is a Novel Regulator of Epithelial-Mesenchymal-Transition in Non-small Cell Lung Cancer.The Journal of biological chemistry · 2015Article
- Matrix stiffness drives epithelial-mesenchymal transition and tumour metastasis through a TWIST1-G3BP2 mechanotransduction pathway.Nature cell biology · 2015Article
- Nuclear ubiquitination by FBXL5 modulates Snail1 DNA binding and stability.Nucleic acids research · 2014Article
- Low osteogenic differentiation potential of placenta-derived mesenchymal stromal cells correlates with low expression of the transcription factors Runx2 and Twist2.Stem cells and development · 2013Article
- Computational modeling of the bHLH domain of the transcription factor TWIST1 and R118C, S144R and K145E mutants.BMC bioinformatics · 2012Article
- Normal and disease-related biological functions of Twist1 and underlying molecular mechanisms.Cell research · 2012Review
- IL-6 stabilizes Twist and enhances tumor cell motility in head and neck cancer cells through activation of casein kinase 2.PloS one · 2011Article
- PKB/AKT phosphorylation of the transcription factor Twist-1 at Ser42 inhibits p53 activity in response to DNA damage.Oncogene · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundTwist is a transcription factor that plays an important role in proliferation and tumorigenesis. Twist is a nuclear protein that regulates a variety of cellular functions controlled by protein-protein interactions and gene transcription events. The focus of this study was to characterize putative nuclear localization signals (NLSs) 37RKRR40 and 73KRGKK77 in the human TWIST (H-TWIST) protein.
resultsUsing site-specific mutagenesis and immunofluorescences, we observed that altered TWISTNLS1 K38R, TWISTNLS2 K73R and K77R constructs inhibit nuclear accumulation of H-TWIST in mammalian cells, while TWISTNLS2 K76R expression was un-affected and retained to the nucleus. Subsequently, co-transfection of TWIST mutants K38R, K73R and K77R with E12 formed heterodimers and restored nuclear localization despite the NLSs mutations. Using a yeast-two-hybrid assay, we identified a novel TWIST-interacting candidate TCF-4, a basic helix-loop-helix transcription factor. The interaction of TWIST with TCF-4 confirmed using NLS rescue assays, where nuclear expression of mutant TWISTNLS1 with co-transfixed TCF-4 was observed. The interaction of TWIST with TCF-4 was also seen using standard immunoprecipitation assays.
conclusionOur study demonstrates the presence of two putative NLS motifs in H-TWIST and suggests that these NLS sequences are functional. Furthermore, we identified and confirmed the interaction of TWIST with a novel protein candidate TCF-4.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.