ArticleCurrent neurovascular research2009
The pro-apoptotic substance thapsigargin selectively stimulates re-growth of brain capillaries.
Article in Current neurovascular research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Organotypic brain slice cultures: A review.Neuroscience · 2015Review
- Organotypic brain slice cultures as a model to study angiogenesis of brain vessels.Frontiers in cell and developmental biology · 2015Article
- Nifedipine and nimodipine protect dopaminergic substantia nigra neurons against axotomy-induced cell death in rat vibrosections via modulating inflammatory responses.Brain research · 2014Article
- Organotypic vibrosections: novel whole sagittal brain cultures.Journal of neuroscience methods · 2011Article
- Hypercholesterolemia in rats impairs the cholinergic system and leads to memory deficits.Molecular and cellular neurosciences · 2010Article
- Effects of cholesterol and its 24S-OH and 25-OH oxysterols on choline acetyltransferase-positive neurons in brain slices.Pharmacology · 2010Article
- Rotenone induces cell death of cholinergic neurons in an organotypic co-culture brain slice model.Neurochemical research · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Thapsigargin is a pro-apoptotic chemical, which has been shown to be useful to study cell death of cholinergic or dopaminergic neurons, or cells, which degenerate in Alzheimer's disease or Parkinson's disease, respectively. The aim of the present work was to study the effects of thapsigargin in the well established organotypic brain co-slice model composed of the basal nucleus of Meynert (nBM), ventral mesencephalon (vMes), dorsal striatum (dStr) and parietal cortex (Ctx). Cholinergic acetyltransferase-positive neurons in the nBM and dStr and dopaminergic tyrosine hydroxylase-positive neurons in the vMes survived, when cultured for 4 weeks with nerve growth factor and glial cell line-derived neurotrophic factor. Nerve fibers of cholinergic nBM neurons grew into the cortex and dopaminergic nerve fibers sprouted into dopamine D2 receptor-positive dStr. The whole co-slice contained a dense laminin-positive capillary network. Treatment of co-cultures with 3 microM thapsigargin for 24 hr significantly decreased the number of cholinergic neurons and dopaminergic neurons. This cell death displayed apoptotic DAPI-positive malformed nuclei and enhanced TUNEL-positive cells. Thapsigargin selectively stimulated the laminin-positive capillary growth between the nBM and Ctx. In conclusion, the induced cell death of cholinergic and dopaminergic neurons may be accompanied by enhanced angiogenic activity.
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