ArticleGenetica2009
Mariner Mos1 transposase optimization by rational mutagenesis.
Article in Genetica, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Mobility of mPing and its associated elements is regulated by both internal and terminal sequences.Mobile DNA · 2023Article
- Artificial optimization of bambooFrontiers in plant science · 2022Article
- Affinities of Terminal Inverted Repeats to DNA Binding Domain of Transposase Affect the Transposition Activity of BambooInternational journal of molecular sciences · 2019Article
- Nuclear export signal (NES) of transposases affects the transposition activity of mariner-like elementsMobile DNA · 2019Article
- Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.Nucleic acids research · 2018Article
- Biochemical characterization and comparison of two closely related active mariner transposases.Biochemistry · 2014Article
- The Drosophila mojavensis Bari3 transposon: distribution and functional characterization.Mobile DNA · 2014Article
- cAMP protein kinase phosphorylates the Mos1 transposase and regulates its activity: evidences from mass spectrometry and biochemical analyses.Nucleic acids research · 2014Article
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- Article
- Bioinformatics and genomic analysis of transposable elements in eukaryotic genomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Nuclear importation of Mariner transposases among eukaryotes: motif requirements and homo-protein interactions.PloS one · 2011Article
- Transposon tools: worldwide landscape of intellectual property and technological developments.Genetica · 2010Review
- Physical properties of DNA components affecting the transposition efficiency of the mariner Mos1 element.Molecular genetics and genomics : MGG · 2009Article
- Nanotechnology approaches for gene transfer.Genetica · 2009Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mariner transposons are probably the most widespread transposable element family in animal genomes. To date, they are believed not to require species-specific host factors for transposition. Despite this, Mos1, one of the most-studied mariner elements (with Himar1), has been shown to be active in insects, but inactive in mammalian genomes. To circumvent this problem, one strategy consists of both enhancing the activity of the Mos1 transposase (MOS1), and making it insensitive to activity-altering post-translational modifications. Here, we report rational mutagenesis studies performed to obtain hyperactive and non-phosphorylable MOS1 variants. Transposition assays in bacteria have made it possible to isolate numerous hyperactive MOS1 variants. The best mutant combinations, named FETY and FET, are 60- and 800-fold more active than the wild-type MOS1 version, respectively. However, there are serious difficulties in using them, notably because they display severe cytotoxicity. On the other hand, three positions lying within the HTH motif, T88, S99, and S104 were found to be sensitive to phosphorylation. Our efforts to obtain active non-phosphorylable mutants at S99 and S104 positions were unsuccessful, as these residues, like the co-linear amino acids in their close vicinity, are critical for MOS1 activity. Even if host factors are not essential for transposition, our data demonstrate that the host machinery is essential in regulating MOS1 activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.