ArticleProtoplasma2009
Transient gene suppression in a red alga, Cyanidioschyzon merolae 10D.
Article in Protoplasma, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Fascinating single-cell red algae: models for evolution and adaptation.The New phytologist · 2026Review
- The Unicellular Red Alga Cyanidioschyzon merolae-The Simplest Model of a Photosynthetic Eukaryote.Plant & cell physiology · 2021Review
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- PEG-mediated, Stable, Nuclear and Chloroplast Transformation ofBio-protocol · 2019Article
- Onsite GTP fuelling via DYNAMO1 drives division of mitochondria and peroxisomes.Nature communications · 2018Article
- Insights into the Mechanisms of Chloroplast Division.International journal of molecular sciences · 2018Review
- Chloramphenicol acetyltransferase-a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae.Protoplasma · 2017Article
- Current status and perspectives of genome editing technology for microalgae.Biotechnology for biofuels · 2017Review
- A nitrogen source-dependent inducible and repressible gene expression system in the red alga Cyanidioschyzon merolae.Frontiers in plant science · 2015Article
- Article
- Development of a heat-shock inducible gene expression system in the red alga Cyanidioschyzon merolae.PloS one · 2014Article
- Article
- Mitotic inheritance of endoplasmic reticulum in the primitive red alga Cyanidioschyzon merolae.Protoplasma · 2012Article
- The early days of plastid retrograde signaling with respect to replication and transcription.Frontiers in plant science · 2012Article
- RNA-mediated silencing in Algae: biological roles and tools for analysis of gene function.Eukaryotic cell · 2011Review
- Division of cell nuclei, mitochondria, plastids, and microbodies mediated by mitotic spindle poles in the primitive red alga Cyanidioschyzon merolae.Protoplasma · 2010Article
- The coiled-coil protein VIG1 is essential for tethering vacuoles to mitochondria during vacuole inheritance of Cyanidioschyzon merolae.The Plant cell · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antisense suppression is a powerful tool to analyze gene function. In this study, we show that antisense RNA suppressed the expression of a target gene in the unicellular red alga, Cyanidioschyzon merolae. In this study, the antisense strand of the catalase gene was cloned and inserted into an expression vector upstream of the GFP gene. This plasmid was introduced into C. merolae cells using a polyethylene glycol-mediated transformation protocol. Using the expression of GFP as a marker of transformed cells, the expression of catalase was examined by immunocytochemistry. Decreased expression of catalase was observed in cells that were transformed with the antisense strand of the catalase gene. These results indicate the utility of this antisense suppression system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.