ArticleIn vitro cellular & developmental biology. Animal2009
Generation of a tumor spheroid in a microgravity environment as a 3D model of melanoma.
Article in In vitro cellular & developmental biology. Animal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Immune System-Tumor Crosstalk Under Microgravity: Mechanistic Insights, Challenges, and Translational Perspectives.Cancers · 2025Review
- Cellular response in three-dimensional spheroids and tissues exposed to real and simulated microgravity: a narrative review.NPJ microgravity · 2024Review
- From simplicity to complexity in current melanoma models.Experimental dermatology · 2022Review
- The Fight against Cancer by Microgravity: The Multicellular Spheroid as a Metastasis Model.International journal of molecular sciences · 2022Review
- Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity.Frontiers in cell and developmental biology · 2022Article
- Employing hydrogels in tissue engineering approaches to boost conventional cancer-based research and therapies.RSC advances · 2021Review
- The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold.Cancers · 2020Article
- Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity.Scientific reports · 2018Article
- Three-dimensional cell culture: A powerful tool in tumor research and drug discovery.Oncology letters · 2017Article
- Application of Hanging Drop Technique for Kidney Tissue Culture.Kidney & blood pressure research · 2017Article
- Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture.Journal of biomedical optics · 2017Article
- A scaffold-free surface culture of B16F10 murine melanoma cells based on magnetic levitation.Cytotechnology · 2016Article
- Scaffold-Free Coculture Spheroids of Human Colonic Adenocarcinoma Cells and Normal Colonic Fibroblasts Promote Tumorigenicity in Nude Mice.Translational oncology · 2016Article
- Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles.Scientific reports · 2015Article
- Three-dimensional in vitro tumor models for cancer research and drug evaluation.Biotechnology advances · 2014Review
- The impact of simulated and real microgravity on bone cells and mesenchymal stem cells.BioMed research international · 2014Review
- 3D spheroids' sensitivity to electric field pulses depends on their size.The Journal of membrane biology · 2013Article
- Cancer research by means of tissue engineering--is there a rationale?Journal of cellular and molecular medicine · 2013Review
- Using space-based investigations to inform cancer research on Earth.Nature reviews. Cancer · 2013Review
- Stiffening of human mesenchymal stem cell spheroid microenvironments induced by incorporation of gelatin microparticles.Journal of the mechanical behavior of biomedical materials · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
An in vitro 3D model was developed utilizing a synthetic microgravity environment to facilitate studying the cell interactions. 2D monolayer cell culture models have been successfully used to understand various cellular reactions that occur in vivo. There are some limitations to the 2D model that are apparent when compared to cells grown in a 3D matrix. For example, some proteins that are not expressed in a 2D model are found up-regulated in the 3D matrix. In this paper, we discuss techniques used to develop the first known large, free-floating 3D tissue model used to establish tumor spheroids. The bioreactor system known as the High Aspect Ratio Vessel (HARVs) was used to provide a microgravity environment. The HARVs promoted aggregation of keratinocytes (HaCaT) that formed a construct that served as scaffolding for the growth of mouse melanoma. Although there is an emphasis on building a 3D model with the proper extracellular matrix and stroma, we were able to develop a model that excluded the use of matrigel. Immunohistochemistry and apoptosis assays provided evidence that this 3D model supports B16.F10 cell growth, proliferation, and synthesis of extracellular matrix. Immunofluorescence showed that melanoma cells interact with one another displaying observable cellular morphological changes. The goal of engineering a 3D tissue model is to collect new information about cancer development and develop new potential treatment regimens that can be translated to in vivo models while reducing the use of laboratory animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.