ArticleLab on a chip2009
Microfluidics for cryopreservation.
Article in Lab on a chip, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- Microfluidic Platforms for Cryopreservation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Supercooling preservation of vascularized composite allografts through CPA optimization, thermal tracking, and stepwise loading techniques.Scientific reports · 2024Article
- Biomedical Applications of Microfluidic Devices: A Review.Biosensors · 2022Review
- Development of an Open Microfluidic Platform for Oocyte One-Stop Vitrification with Cryotop Method.Biosensors · 2022Article
- Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer.Micromachines · 2022Article
- Cryopreservation of NK and T Cells Without DMSO for Adoptive Cell-Based Immunotherapy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Advanced technologies for the preservation of mammalian biospecimens.Nature biomedical engineering · 2021Review
- Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- On-chip oocyte denudation from cumulus-oocyte complexes for assisted reproductive therapy.Lab on a chip · 2018Article
- Biomanufacturing for clinically advanced cell therapies.Nature biomedical engineering · 2018Review
- [Effect of cryoprotectant removal by microfluidic chip on developmental capacity of oocytes].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2018Article
- Geometrically-mediated snap-off of water-in-oil emulsion droplets in microfluidic flow focusing devices.Journal of oil, gas and petrochemical sciences · 2018Article
- Bio-inspired solute enables preservation of human oocytes using minimum volume vitrification.Journal of tissue engineering and regenerative medicine · 2018Article
- Application of microfluidic technologies to human assisted reproduction.Molecular human reproduction · 2017Review
- A microfluidic perfusion approach for on-chip characterization of the transport properties of human oocytes.Lab on a chip · 2017Article
- Synergistic Development of Biochips and Cell Preservation Methodologies: A Tale of Converging Technologies.Current stem cell reports · 2017Article
- Portable microfluidic integrated plasmonic platform for pathogen detection.Scientific reports · 2015Article
- Slow and steady cell shrinkage reduces osmotic stress in bovine and murine oocyte and zygote vitrification.Human reproduction (Oxford, England) · 2015Article
- Article
- Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation.Biomedical microdevices · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Minimizing cell damage throughout the cryopreservation process is critical to enhance the overall outcome. Osmotic shock sustained during the loading and unloading of cryoprotectants (CPAs) is a major source of cell damage during the cryopreservation process. We introduce a microfluidic approach to minimize osmotic shock to cells during cryopreservation. This approach allows us to control the loading and unloading of CPAs in microfluidic channels using diffusion and laminar flow. We provide a theoretical explanation of how the microfluidic approach minimizes osmotic shock in comparison to conventional cryopreservation protocols via cell membrane transport modeling. Finally, we show that biological experiments are consistent with the proposed mathematical model. The results indicate that our novel microfluidic-based approach improves post-thaw cell survivability by up to 25% on average over conventional cryopreservation protocols. The method developed in this study provides a platform to cryopreserve cells with higher viability, functionality, and minimal inter-technician variability. This method introduces microfluidic technologies to the field of biopreservation, opening the door to future advancements at the interface of these fields.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.