ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
High-efficiency transient transduction of human embryonic stem cell-derived neurons with baculoviral vectors.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Fluorescence-based quantification of mitochondrial damage in human airway smooth muscle cells.American journal of physiology. Cell physiology · 2026Article
- TNFα-mediated subcellular heterogeneity of succinate dehydrogenase activity in human airway smooth muscle cells.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Heterogeneous distribution of mitochondria and succinate dehydrogenase activity in human airway smooth muscle cells.FASEB bioAdvances · 2024Article
- Cell-Based Measurement of Mitochondrial Function in Human Airway Smooth Muscle Cells.International journal of molecular sciences · 2023Article
- Megalin mediates plasma membrane to mitochondria cross-talk and regulates mitochondrial metabolism.Cellular and molecular life sciences : CMLS · 2018Article
- Production and Purification of Baculovirus for Gene Therapy Application.Journal of visualized experiments : JoVE · 2018Article
- Gene Delivery Approaches for Mesenchymal Stem Cell Therapy: Strategies to Increase Efficiency and Specificity.Stem cell reviews and reports · 2017Review
- Transgene expression in Penaeus monodon cells: evaluation of recombinant baculoviral vectors with shrimp specific hybrid promoters.Cytotechnology · 2016Article
- Purification of baculovirus vectors using heparin affinity chromatography.Molecular therapy. Methods & clinical development · 2016Article
- Manufacturing of AcMNPV baculovirus vectors to enable gene therapy trials.Molecular therapy. Methods & clinical development · 2016Review
- Efficient gene delivery into cell lines and stem cells using baculovirus.Nature protocols · 2014Article
- Antitumor effects of CD40 ligand-expressing endothelial progenitor cells derived from human induced pluripotent stem cells in a metastatic breast cancer model.Stem cells translational medicine · 2014Article
- Genetic rearrangements of variable di-residue (RVD)-containing repeat arrays in a baculoviral TALEN system.Molecular therapy. Methods & clinical development · 2014Article
- Systemic delivery of fusogenic membrane glycoprotein-expressing neural stem cells to selectively kill tumor cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Baculovirus: an insect-derived vector for diverse gene transfer applications.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Review
- Targeted suicide gene therapy for glioma using human embryonic stem cell-derived neural stem cells genetically modified by baculoviral vectors.Gene therapy · 2012Article
- Baculovirus-transduced bone marrow mesenchymal stem cells for systemic cancer therapy.Cancer gene therapy · 2010Article
- Baculovirus as a gene delivery vector: recent understandings of molecular alterations in transduced cells and latest applications.Biotechnology advancesReview
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Authors and funding
6 authors.
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Abstract
Transient genetic manipulation of human neurons without chromosomal integration of the transgene would be valuable but has been challenging due to the quiescent nature of these postmitotic cells. In this study, we developed a set of baculoviral vectors for transient transduction in nondividing neurons derived from human embryonic stem cells (hESCs). Using a baculoviral vector equipped with the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), we observed a quick onset of transgene expression as early as day 1 after baculoviral transduction and a high efficiency of up to 80%. Strong transgene expression in the cultured human neurons was observed for more than 1 month and the signal was easily detectable even after 3 months. Using two baculoviral vectors carrying different transgenes, we found that co-transduction at a single neuron level was possible. After transplantation into the brain of nude mice, the baculovirus-transduced human neurons were integrated into the mouse brain and maintained transgene expression for at least 4 weeks, portending the usefulness of this technique in assisting neural transplantation. Therefore, by mediating efficient transient gene expression, baculoviral vectors can provide useful tools for both basic gene function studies in human neurons and therapeutic applications of these cells.
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