ArticleNucleic acids research2009
The Myb/SANT domain of the telomere-binding protein TRF2 alters chromatin structure.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Review
- Structural biology of shelterin and telomeric chromatin: the pieces and an unfinished puzzle.Biochemical Society transactions · 2024Review
- TRF1 and TRF2: pioneering targets in telomere-based cancer therapy.Journal of cancer research and clinical oncology · 2024Review
- Spotlights on adult patients with pediatric-type diffuse gliomas in accordance with the 2021 WHO classification of CNS tumors.Frontiers in neuroscience · 2023Article
- Abnormal function of telomere protein TRF2 induces cell mutation and the effects of environmental tumor‑promoting factors (Review).Oncology reports · 2021Review
- Human telomerase is directly regulated by non-telomeric TRF2-G-quadruplex interaction.Cell reports · 2021Article
- First interspecific genetic linkage map for Castanea sativa x Castanea crenata revealed QTLs for resistance to Phytophthora cinnamomi.PloS one · 2017Article
- TRF2-Mediated Control of Telomere DNA Topology as a Mechanism for Chromosome-End Protection.Molecular cell · 2016Article
- Identification of putative interactions between swine and human influenza A virus nucleoprotein and human host proteins.Virology journal · 2014Article
- The centromere: epigenetic control of chromosome segregation during mitosis.Cold Spring Harbor perspectives in biology · 2014Review
- Telomeric repeats act as nucleosome-disfavouring sequences in vivo.Nucleic acids research · 2014Article
- The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.FEBS open bio · 2014Article
- TRF2 controls telomeric nucleosome organization in a cell cycle phase-dependent manner.PloS one · 2012Article
- The linker region of macroH2A promotes self-association of nucleosomal arrays.The Journal of biological chemistry · 2011Article
- Article
- Myb proteins: angels and demons in normal and transformed cells.Frontiers in bioscience (Landmark edition) · 2011Review
- The human telomeric protein hTRF1 induces telomere-specific nucleosome mobility.Nucleic acids research · 2010Article
- Conservation of telomere protein complexes: shuffling through evolution.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Eukaryotic DNA is packaged into chromatin, which regulates genome activities such as telomere maintenance. This study focuses on the interactions of a myb/SANT DNA-binding domain from the telomere-binding protein, TRF2, with reconstituted telomeric nucleosomal array fibers. Biophysical characteristics of the factor-bound nucleosomal arrays were determined by analytical agarose gel electrophoresis (AAGE) and single molecules were visualized by atomic force microscopy (AFM). The TRF2 DNA-binding domain (TRF2 DBD) neutralized more negative charge on the surface of nucleosomal arrays than histone-free DNA. Binding of TRF2 DBD at lower concentrations increased the radius and conformational flexibility, suggesting a distortion of the fiber structure. Additional loading of TRF2 DBD onto the nucleosomal arrays reduced the flexibility and strongly blocked access of micrococcal nuclease as contour lengths shortened, consistent with formation of a unique, more compact higher-order structure. Mirroring the structural results, TRF2 DBD stimulated a strand invasion-like reaction, associated with telomeric t-loops, at lower concentrations while inhibiting the reaction at higher concentrations. Full-length TRF2 was even more effective at stimulating this reaction. The TRF2 DBD had less effect on histone-free DNA structure and did not stimulate the t-loop reaction with this substrate, highlighting the influence of chromatin structure on the activities of DNA-binding proteins.
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