ArticleMolecular genetics and genomics : MGG2009
Functional analysis and subcellular localization of two geranylgeranyl diphosphate synthases from Penicillium paxilli.
Article in Molecular genetics and genomics : MGG, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 37 citations in OpenAlex.
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- Subcellular localization of fungal specialized metabolites.Fungal biology and biotechnology · 2022Review
- The Mevalonate Pathway Is Important for Growth, Spore Production, and the Virulence ofFrontiers in microbiology · 2021Article
- Tremorgenic and neurotoxic paspaline-derived indole-diterpenes: biosynthetic diversity, threats and applications.Applied microbiology and biotechnology · 2019Review
- Pex14/17, a filamentous fungus-specific peroxin, is required for the import of peroxisomal matrix proteins and full virulence of Magnaporthe oryzae.Molecular plant pathology · 2017Article
- Draft Genome Sequence of the Filamentous Fungus Penicillium paxilli (ATCC 26601).Genome announcements · 2015Article
- Spatial and temporal control of fungal natural product synthesis.Natural product reports · 2014Review
- Co-ordination between BrlA regulation and secretion of the oxidoreductase FmqD directs selective accumulation of fumiquinazoline C to conidial tissues in Aspergillus fumigatus.Cellular microbiology · 2014Article
- Deletion and gene expression analyses define the paxilline biosynthetic gene cluster in Penicillium paxilli.Toxins · 2013Article
- Expanding functional repertoires of fungal peroxisomes: contribution to growth and survival processes.Frontiers in physiology · 2013Article
- The peroxisome: an update on mysteries.Histochemistry and cell biology · 2012Review
- Subcellular evidence for the involvement of peroxisomes in plant isoprenoid biosynthesis.Plant signaling & behavior · 2011Article
- The significance of peroxisomes in secondary metabolite biosynthesis in filamentous fungi.Biotechnology letters · 2011Review
- Compartmentalization and molecular traffic in secondary metabolism: a new understanding of established cellular processes.Fungal genetics and biology : FG & B · 2011Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The filamentous fungus Penicillium paxilli contains two distinct geranylgeranyl diphosphate (GGPP) synthases, GgsA and GgsB (PaxG). PaxG and its homologues in Neotyphodium lolii and Fusarium fujikuroi are associated with diterpene secondary metabolite gene clusters. The genomes of other filamentous fungi including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae and Fusarium graminearum also contain two or more copies of GGPP synthase genes, although the diterpene metabolite capability of these fungi is not known. The objective of this study was to understand the biological significance of the presence of two copies of GGPP synthases in P. paxilli by investigating their subcellular localization. Using a carotenoid complementation assay and gene deletion analysis, we show that P. paxilli GgsA and PaxG have GGPP synthase activities and that paxG is required for paxilline biosynthesis, respectively. In the DeltapaxG mutant background ggsA was unable to complement paxilline biosynthesis. A GgsA-EGFP fusion protein was localized to punctuate organelles and the EGFP-GRV fusion protein, containing the C-terminus tripeptide GRV of PaxG, was localized to peroxisomes. A truncated PaxG mutant lacking the C-terminus tripeptide GRV was unable to complement a DeltapaxG mutant demonstrating that the tripeptide is functionally important for paxilline biosynthesis.
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