ArticleJournal of applied crystallography2008
Cryocrystallography in capillaries: critical glycerol concentrations and cooling rates.
Article in Journal of applied crystallography, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Automated device for rapid sample cooling via controlled submersion.Cryobiology · 2025Article
- Ultra-Rapid Laser Calorimetry for the Assessment of Crystallization in Low-Concentration Cryoprotectants.Journal of heat transfer · 2022Article
- Article
- Cryopreservation of NK and T Cells Without DMSO for Adoptive Cell-Based Immunotherapy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Ice in biomolecular cryocrystallography.Acta crystallographica. Section D, Structural biology · 2021Article
- Density and electron density of aqueous cryoprotectant solutions at cryogenic temperatures for optimized cryoprotection and diffraction contrast.Acta crystallographica. Section D, Structural biology · 2018Article
- A standardized technique for high-pressure cooling of protein crystals.Acta crystallographica. Section D, Structural biology · 2017Article
- Crystal Dehydration in Membrane Protein Crystallography.Advances in experimental medicine and biology · 2016Article
- Practical macromolecular cryocrystallography.Acta crystallographica. Section F, Structural biology communications · 2015Review
- Improved reproducibility of unit-cell parameters in macromolecular cryocrystallography by limiting dehydration during crystal mounting.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Breaking the radiation damage limit with Cryo-SAXS.Biophysical journal · 2013Article
- Effect of common cryoprotectants on critical warming rates and ice formation in aqueous solutions.Cryobiology · 2012Article
- Crystalline ice as a cryoprotectant: theoretical calculation of cooling speed in capillary tubes.Journal of microscopy · 2011Article
- Slow cooling and temperature-controlled protein crystallography.Journal of structural and functional genomics · 2010Article
- Slow cooling of protein crystals.Journal of applied crystallography · 2009Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Capillary tubes have many advantages over multi-well plates for macromol-ecular crystal growth and handling, including the possibility of in situ structure determination. To obtain complete high-resolution X-ray data sets, cryopreservation protocols must be developed to prevent crystalline ice formation and preserve macromolecular crystal order. The minimum glycerol concentrations required to vitrify aqueous solutions during plunging into liquid nitrogen and liquid propane have been determined for capillary diameters from 3.3 mm to 150 microm. For the smallest diameter, the required glycerol concentrations are 30%(w/v) in nitrogen and 20%(w/v) in propane, corresponding to cooling rates of approximately 800 and approximately 7000 K s(-1), respectively. These concentrations are much larger than are required in current best practice using crystals in loops or on microfabricated mounts. In additon, the relation between the minimum cooling rate for vitrification and glycerol concentration has been estimated; this relation is of fundamental importance in developing rational cryopreservation protocols.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.