ArticleMolecular plant2009
Immunoelectron microscopy for locating calvin cycle enzymes in the thylakoids of synechocystis 6803.
Article in Molecular plant, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 36 citations in OpenAlex.
- Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803.Plant physiology · 2026Article
- Optimal energy and redox metabolism in the cyanobacterium Synechocystis sp. PCC 6803.NPJ systems biology and applications · 2023Article
- Loss of Chloroplast GNAT Acetyltransferases Results in Distinct Metabolic Phenotypes in Arabidopsis.Plant & cell physiology · 2023Article
- Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat.Frontiers in plant science · 2023Article
- Elucidation of the coping strategy in an OMP homozygous knockout mutant of Synechocystis 6803 defective in iron uptake.Archives of microbiology · 2022Article
- Integrating on-grid immunogold labeling and cryo-electron tomography to reveal photosystem II structure and spatial distribution in thylakoid membranes.Journal of structural biology · 2021Article
- Probing Light-Dependent Regulation of the Calvin Cycle Using a Multi-Omics Approach.Frontiers in plant science · 2021Article
- Cyanobacterial carboxysome mutant analysis reveals the influence of enzyme compartmentalization on cellular metabolism and metabolic network rigidity.Metabolic engineering · 2019Article
- Light Modulates the Biosynthesis and Organization of Cyanobacterial Carbon Fixation Machinery through Photosynthetic Electron Flow.Plant physiology · 2016Article
- Regulation of CO2 Concentrating Mechanism in Cyanobacteria.Life (Basel, Switzerland) · 2015Review
- Identification and characterization of a carboxysomal γ-carbonic anhydrase from the cyanobacterium Nostoc sp. PCC 7120.Photosynthesis research · 2014Article
- Differential distribution of pigment-protein complexes in the Thylakoid membranes of Synechocystis 6803.Journal of bioenergetics and biomembranes · 2012Article
- Quantitative evaluation of freeze-substitution effects on preservation of nuclear antigens during preparation of biological samples for immunoelectron microscopy.Histochemistry and cell biology · 2012Article
- Article
- Modulation of the major paths of carbon in photorespiratory mutants of synechocystis.PloS one · 2011Article
- Restoration of photosystem II photochemistry and carbon assimilation and related changes in chlorophyll and protein contents during the rehydration of desiccated Xerophyta scabrida leaves.Journal of experimental botany · 2011Article
- Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unicellular cyanobacteria Synechocystis 6803 were fixed using high-pressure freezing (HPF) and freeze substitution without any chemical cross-linkers. Immunoelectron microscopy of these cells showed that five sequential enzymes of the Calvin cycle (phosphoriboisomerase, phosphoribulokinase, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), 3-phosphoglyceratekinase and glyceraldehyde-3-phosphate dehydrogenase) and the catalytic portion of the chloroplast H+-ATP synthase (CF1) are located adjacent to the thylakoid membranes. Cell-free extracts of Synechocystis were processed by ultracentrifugation to isolate thylakoid fractions sedimenting at 40,000, 90,000, and 150,000 g. Among these, the 150,000-g fraction showed the highest linked activity of the above five sequential Calvin cycle enzymes and also the highest coordinated activity of light and dark reactions as assessed by ribose-5-phosphate (R-5-P) +ADP dependent CO2 fixation. Immunogold labeling of this membrane fraction confirmed the presence of the above five enzymes as well as the catalytic portion of the CF1 ATP synthase. Notably, the protein A-gold labeling of the thylakoids was observed without use of chemical cross-linkers and in spite of the normal washing steps used during standard immunolabeling. The results showed that soluble Calvin cycle enzymes might be organized along the thylakoid membranes.
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