ArticleJournal of applied crystallography2009
Facilitating protein crystal cryoprotection in thick-walled plastic capillaries by high-pressure cryocooling.
Article in Journal of applied crystallography, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- Practical macromolecular cryocrystallography.Acta crystallographica. Section F, Structural biology communications · 2015Review
- A high-pressure cryocooling method for protein crystals and biological samples with reduced background X-ray scatter.Journal of applied crystallography · 2013Article
- Fast high-pressure freezing of protein crystals in their mother liquor.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Microcrystallography, high-pressure cryocooling and BioSAXS at MacCHESS.Journal of synchrotron radiation · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Many steps in the X-ray crystallographic solution of protein structures have been automated. However, the harvesting and cryocooling of crystals still rely primarily on manual handling, frequently with consequent mechanical damage. An attractive alternative is to grow crystals directly inside robust plastic capillaries that may be cryocooled and mounted on the beamline goniometer. In this case, it is still desirable to devise a way to cryoprotect the crystals, which is difficult owing to the poor thermal conductivity of thick plastic capillary walls and the large thermal mass of the capillary and internal mother liquor. A method is described to circumvent these difficulties. It is shown that high-pressure cryocooling substantially reduced the minimal concentrations of cryoprotectants required to cryocool water inside capillaries without formation of ice crystals. The minimal concentrations of PEG 200, PEG 400 and glycerol necessary for complete vitrification under pressure cryocooling were determined.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.