ArticleGenetics2009
High-throughput multiplex sequencing to discover copy number variants in Drosophila.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Segmental duplications are hot spots of copy number variants affecting barley gene content.The Plant journal : for cell and molecular biology · 2020Article
- Bayesian localization of CNV candidates in WGS data within minutes.Algorithms for molecular biology : AMB · 2019Article
- SeqCNV: a novel method for identification of copy number variations in targeted next-generation sequencing data.BMC bioinformatics · 2017Article
- Notch activation as a driver of osteogenic sarcoma.Cancer cell · 2014Article
- Copy number polymorphism in plant genomes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Review
- Chromosomal structural variations during progression of a prostate epithelial cell line to a malignant metastatic state inactivate the NF2, NIPSNAP1, UGT2B17, and LPIN2 genes.Cancer biology & therapy · 2013Article
- De novo characterization of Larix gmelinii (Rupr.) Rupr. transcriptome and analysis of its gene expression induced by jasmonates.BMC genomics · 2013Article
- A reduced representation approach to population genetic analyses and applications to human evolution.Genome research · 2011Article
- Structural variation in the chicken genome identified by paired-end next-generation DNA sequencing of reduced representation libraries.BMC genomics · 2011Article
- Combining target enrichment with barcode multiplexing for high throughput SNP discovery.BMC genomics · 2010Article
- Using next-generation sequencing for high resolution multiplex analysis of copy number variation from nanogram quantities of DNA from formalin-fixed paraffin-embedded specimens.Nucleic acids research · 2010Article
- Population genomic inferences from sparse high-throughput sequencing of two populations of Drosophila melanogaster.Genome biology and evolution · 2009Article
- Characterizing natural variation using next-generation sequencing technologies.Trends in genetics : TIG · 2009Review
- Single-molecule sequencing of an individual human genome.Nature biotechnology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Copy number variation (CNV) contributes in phenotypically relevant ways to the genetic variability of many organisms. Cost-effective genomewide methods for identifying copy number variation are necessary to elucidate the contribution that these structural variants make to the genomes of model organisms. We have developed a novel approach for the identification of copy number variation by next generation sequencing. As a proof of concept our method has been applied to map the deletions of three Drosophila deficiency strains. We demonstrate that low sequence coverage is sufficient for identifying and mapping large deletions at kilobase resolution, suggesting that data generated from high-throughput sequencing experiments are sufficient for simultaneously analyzing many strains. Genomic DNA from two Drosophila deficiency stocks was barcoded and sequenced in multiplex, and the breakpoints associated with each deletion were successfully identified. The approach we describe is immediately applicable to the systematic exploration of copy number variation in model organisms and humans.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.