ArticleAntimicrobial agents and chemotherapy2009
Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids.
Article in Antimicrobial agents and chemotherapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed.
- Enhanced metagenomic surveillance for bovine respiratory disease pathogens and antimicrobial resistance by hybridization capture sequencing.Applied and environmental microbiology · 2025Article
- Communities of plasmids as strategies for antimicrobial resistance gene survival in wastewater treatment plant effluent.npj antimicrobials and resistance · 2025Article
- Comparative Analysis of Phenotypic and Genotypic Antibiotic Susceptibility ofAntibiotics (Basel, Switzerland) · 2025Article
- Unveiling resistance patterns, kmt1 sequence analyses, virulence traits, and antibiotic resistance genes of multidrug-resistant Pasteurella multocida retrieved from poultry and rabbits.Scientific reports · 2025Article
- Evolutionary rescue of bacterial populations by heterozygosity on multicopy plasmids.Journal of mathematical biology · 2025Article
- Genomic insights into diversity, antimicrobial resistance and virulence ofFrontiers in microbiology · 2025Article
- Phenotypical and genotypical resistance testing ofFrontiers in veterinary science · 2025Article
- Antimicrobial Susceptibility Profiles ofVeterinary sciences · 2024Article
- Identification and evolution of ICE-PmuST394: a novel integrative conjugative element in Pasteurella multocida ST394.The Journal of antimicrobial chemotherapy · 2024Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
- Article
- Antimicrobial Susceptibility and Resistance Mechanisms inAnimals : an open access journal from MDPI · 2023Article
- Article
- Comparative genomic analysis of Colistin resistant Escherichia coli isolated from pigs, a human and wastewater on colistin withdrawn pig farm.Scientific reports · 2023Article
- Virulence attitude estimation of Pasteurella multocida isolates in embryonated chicken eggs.Archives of microbiology · 2021Article
- Evolution of ColE1-like plasmids across γ-Proteobacteria: From bacteriocin production to antimicrobial resistance.PLoS genetics · 2021Article
- First Emergence of Resistance to Macrolides and Tetracycline Identified inMicroorganisms · 2021Article
- Virulence Determinants and Antimicrobial Profiles ofAntibiotics (Basel, Switzerland) · 2021Article
- Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread inFrontiers in microbiology · 2021Article
- Prevalence and Risk Factors Associated With Antimicrobial Resistance in Bacteria Related to Bovine Respiratory Disease-A Broad Cross-Sectional Study of Beef Cattle at Entry Into Canadian Feedlots.Frontiers in veterinary science · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most gram-negative bacteria, acquired multiresistance is conferred by large plasmids compiling numerous antimicrobial resistance genes. Here, we show an evolutionary alternative strategy used by Pasteurella multocida to become resistant to multiple clinically relevant antibiotics. Thirteen beta-lactam-resistant clinical isolates, concomitantly resistant to tetracyclines and/or streptomycin as well as to sulfonamides, were studied. Pulsed-field gel electrophoresis analysis revealed different profiles among the isolates, showing that clonal dissemination was not the sole event responsible for the spread of multiresistance. Each P. multocida strain carried two or three small plasmids between 4 and 6 kb in size. A direct association between resistance profile and plasmid content was found. Complete nucleotide sequencing of all plasmids revealed seven different replicons, six of them belonging to the ColE1 superfamily. All plasmids carried one, or a maximum of two, antimicrobial resistance determinants. Plasmids pB1000 and pB1002 bore bla(ROB-1), pB1001 carried tet(B), pB1003 and pB1005 carried sul2 and strA, pB1006 harbored tet(O), and p9956 bore the tet(H) gene. All plasmids except pB1002 and pB1006 were successfully transformed into Escherichia coli. pB1000, also involved in beta-lactam resistance in Haemophilus parasuis (A. San Millan et al., Antimicrob. Agents Chemother. 51:2260-2264, 2007), was mobilized in E. coli using the conjugation machinery of an IncP plasmid. Stability experiments proved that pB1000 was stable in P. multocida but highly unstable in E. coli. In conclusion, bla(ROB-1) is responsible for beta-lactam resistance in P. multocida in Spain. Coexistence and the spread of small plasmids are used by P. multocida to become multiresistant.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.