ArticleBiophysical journal2009
Improved model of fluorescence recovery expands the application of multiphoton fluorescence recovery after photobleaching in vivo.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow.Journal of biomedical optics · 2023Article
- Review
- Article
- The role of the lymphatic system in inflammatory-erosive arthritis.Seminars in cell & developmental biology · 2015Review
- Single- and two-photon fluorescence recovery after photobleaching.Cold Spring Harbor protocols · 2015Article
- In vivo quantification of lymph viscosity and pressure in lymphatic vessels and draining lymph nodes of arthritic joints in mice.The Journal of physiology · 2014Article
- Measurement of three-dimensional anisotropic diffusion by multiphoton fluorescence recovery after photobleaching.Annals of biomedical engineering · 2014Article
- FRAP in pharmaceutical research: practical guidelines and applications in drug delivery.Pharmaceutical research · 2014Review
- Measuring intranodal pressure and lymph viscosity to elucidate mechanisms of arthritic flare and therapeutic outcomes.Annals of the New York Academy of Sciences · 2011Article
- Multiphoton fluorescence recovery after photobleaching in bounded systems.Physical review. E, Statistical, nonlinear, and soft matter physics · 2011Article
- Measuring diffusion coefficients via two-photon fluorescence recovery after photobleaching.Journal of visualized experiments : JoVE · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiphoton fluorescence recovery after photobleaching is a well-established microscopy technique used to measure the diffusion of macromolecules in biological systems. We have developed an improved model of the fluorescence recovery that includes the effects of convective flows within a system. We demonstrate the validity of this two-component diffusion-convection model through in vitro experimentation in systems with known diffusion coefficients and known flow speeds, and show that the diffusion-convection model broadens the applicability of the multiphoton fluorescence recovery after photobleaching technique by enabling accurate determination of the diffusion coefficient, even when significant flows are present. Additionally, we find that this model allows for simultaneous measurement of the flow speed in certain regimes. Finally, we demonstrate the effectiveness of the diffusion-convection model in vivo by measuring the diffusion coefficient and flow speed within tumor vessels of 4T1 murine mammary adenocarcinomas implanted in the dorsal skinfold chamber.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.